Ion-Based Proteome-Integrated Solubility Alteration Assays for Systemwide Profiling of Protein–Molecule Interactions
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DOI: 10.1021/acs.analchem.2c00391
Abstract: Unbiased drug target engagement deconvolution and mechanism of action elucidation are major challenges in drug development. Modification-free target engagement methods, such as thermal proteome profiling, have gained increasing popularity in the last several years. However,… read more here.
Keywords: solubility; proteome integrated; integrated solubility; solubility alteration ... See more keywords
System-Wide Profiling by Proteome Integral Solubility Alteration Assay of Drug Residence Times for Target Characterization
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DOI: 10.1101/2022.06.27.497697
Abstract: Most drugs used in the clinic and drug candidates target multiple proteins, and thus detailed characterization of their efficacy targets is required. While current methods rely on quantitative measurements at thermodynamic equilibrium, kinetic parameters such… read more here.
Keywords: proteome integral; residence; solubility alteration; integral solubility ... See more keywords