Soluble expression, purification and biochemical characterization of a C-7 cholesterol dehydrogenase from Drosophila melanogaster
Sign Up to like & getrecommendations! Published in 2019 at "Steroids"
DOI: 10.1016/j.steroids.2019.108495
Abstract: The C-7 cholesterol dehydrogenase (NVD), which converts cholesterol to 7-dehydrocholesterol (7-DHC) by 7,8-dehydrogenation, plays a pivotal role in the metabolism of cholesterol and steroid intermediates. The NVD protein was successfully expressed in insect Sf9 cells.… read more here.
Keywords: cholesterol; cholesterol dehydrogenase; soluble expression; expression purification ... See more keywords
Integrated bioprocessing and genetic strategies to enhance soluble expression of anti-HER2 immunotoxin in E. Coli
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DOI: 10.1186/s13568-024-01765-6
Abstract: Immunotoxins are widely applied for cancer therapy. However, bacterial expression of immunotoxins usually leads to the formation of insoluble and non-functional recombinant proteins. This study was aimed to improve soluble expression of a novel anti-HER2… read more here.
Keywords: soluble expression; immunotoxin; anti her2; her2 immunotoxin ... See more keywords
A “Pro-Asp-Thr” Amino Acid Repeat from Vibrio sp. QY108 Alginate Lyase Exhibits Alginate-Binding Capacity and Enhanced Soluble Expression and Thermostability
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DOI: 10.3390/ijms25115801
Abstract: Alginate lyases cleave the 1,4-glycosidic bond of alginate by eliminating sugar molecules from its bond. While earlier reported alginate lyases were primarily single catalytic domains, research on multi-module alginate lyases has been lfiguimited. This study… read more here.
Keywords: alginate; soluble expression; vibrio qy108; alginate lyase ... See more keywords