A Protocol to Enhance Soluble Protein Expression in the Cytoplasm of Bacillus subtilis.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1859-2_14
Abstract: Bacillus subtilis is a generally regarded as safe (GRAS) microorganism, which has been used for industrial production of recombinant enzymes. Many inducible and inducer-free expression vectors have been developed for intracellular production; some of those… read more here.
Keywords: expression; protein expression; soluble protein; bacillus subtilis ... See more keywords
High-Throughput Isolation of Soluble Protein Domains Using a Bipartite Split-GFP Complementation System.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9624-7_15
Abstract: The identification of soluble, folded domains of proteins is a recurring task in modern molecular biology. We detail a protocol for identifying compact soluble protein domains using a self-assembling two-part split-GFP comprised of a detector… read more here.
Keywords: protein; split gfp; soluble protein; domains using ... See more keywords
Genome-wide association mapping for protein, oil and water-soluble protein contents in soybean
Sign Up to like & getrecommendations! Published in 2020 at "Molecular Genetics and Genomics"
DOI: 10.1007/s00438-020-01704-7
Abstract: As a globally important legume crop, soybean provides excellent sources of protein and oil for human and livestock nutrition. Improving seed protein and oil contents has always been an important objective in soybean breeding. Water-soluble… read more here.
Keywords: protein; soluble protein; oil water; water soluble ... See more keywords
Functional properties of Chinese yam (Dioscorea opposita Thunb. cv. Baiyu) soluble protein
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Food Science and Technology"
DOI: 10.1007/s13197-017-2948-2
Abstract: In this study, yam soluble protein (YSP) was extracted from Chinese yam (Dioscorea opposita Thunb. cv. Baiyu) and the functional properties were investigated under the influence of pH and ionic strength. As results, YSP was… read more here.
Keywords: functional properties; soluble protein; ysp; dioscorea opposita ... See more keywords
Effect of micellar κ-casein dissociation on the formation of soluble protein complexes and acid gel properties
Sign Up to like & getrecommendations! Published in 2018 at "Lwt - Food Science and Technology"
DOI: 10.1016/j.lwt.2017.10.018
Abstract: Abstract This study investigated the effect of micellar κ-casein dissociation, caused by cross-linking agent glutaraldehyde, on the formation of soluble protein complexes and the texture of resulting gels. Reconstituted skim milk containing different levels of… read more here.
Keywords: acid gel; casein; soluble protein; protein complexes ... See more keywords
Effect of heat treatment on physicochemical state and in vitro digestion of salt-soluble protein from Pacific oyster (Crassostrea gigas)
Sign Up to like & getrecommendations! Published in 2020 at "Lwt - Food Science and Technology"
DOI: 10.1016/j.lwt.2020.110126
Abstract: Abstract The current research extracted oyster salt-soluble protein (OSSP) from Pacific oyster (Crassostrea gigas) and investigated the effect of heat treatment (35–100 °C) on the physicochemical state and in vitro digestion of OSSP. Gel electrophoresis results… read more here.
Keywords: oyster crassostrea; soluble protein; pacific oyster; effect heat ... See more keywords
Conversion of a soluble protein into a potent chaperone in vivo
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-39158-6
Abstract: Molecular chaperones play an important role in cellular protein-folding assistance and aggregation inhibition. As a different but complementary model, we previously proposed that, in general, soluble cellular macromolecules with large excluded volume and surface charges… read more here.
Keywords: protein; conversion soluble; soluble protein; chaperone activity ... See more keywords
Recovery of Yam Soluble Protein from Yam Starch Processing Wastewater
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-62372-6
Abstract: Over the past two decades, many studies have shown that the yam storage protein dioscorin, which is abundant in the wastewater of starch processing, exhibits many biological activities both in vitro and in vivo .… read more here.
Keywords: protein; starch processing; soluble protein; recovery yam ... See more keywords
Hydrolysing the soluble protein secreted by Escherichia coli in trans-4-hydroxy-L-proline fermentation increased dissolve oxygen to promote high-level trans-4-hydroxy-L-proline production
Sign Up to like & getrecommendations! Published in 2019 at "Bioengineered"
DOI: 10.1080/21655979.2019.1600966
Abstract: ABSTRACT Trans-4-hydroxy-L-proline (Hyp) production by Escherichia coli (E. coli) in fermentation is a high-oxygen-demand process. E. coli secretes large amounts of soluble protein, especially in the anaphase of fermentation, which is an important factor leading… read more here.
Keywords: soluble protein; production; fermentation; hyp ... See more keywords
Bactericidal mechanisms and effector targets of TiO2 and Ag-TiO2 against Staphylococcus aureus
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Medical Microbiology"
DOI: 10.1099/jmm.0.000457
Abstract: Purpose. In our previous study, Ag+‐loaded TiO2 and Ag+‐loaded SiO2 coatings for tracheal intubation were prepared to prevent ventilator‐associated pneumonia (VAP), but the antimicrobial targets and the underlying mechanisms of TiO2 and Ag‐TiO2 (Ag+) are… read more here.
Keywords: tio2; tio2 tio2; staphylococcus aureus; soluble protein ... See more keywords
The impact of wounding and postharvest storage conditions on retention of soluble protein in sugar beet leaves.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of food science"
DOI: 10.1111/1750-3841.16512
Abstract: Sugar beet leaves can be a viable and economically interesting source of high-quality protein for the food industry. We investigated how storage conditions and leaf wounding at harvest affect the content and quality of the… read more here.
Keywords: beet leaves; sugar beet; soluble protein; storage ... See more keywords