Expression and Purification of a Functional E. coli 13CH3-Methionine-Labeled Thermostable Neurotensin Receptor 1 Variant for Solution NMR Studies.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9121-1_3
Abstract: Escherichia coli (E. coli) is the most widely used expression host for recombinant proteins due to high expression yields and straightforward molecular cloning. Directed evolution of G protein-coupled receptors (GPCRs) has made several of these… read more here.
Keywords: neurotensin receptor; solution nmr; methionine; 13ch3 methionine ... See more keywords
Partial alignment, residual dipolar couplings and molecular symmetry in solution NMR.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of biomolecular NMR"
DOI: 10.1007/s10858-019-00256-2
Abstract: Residual dipolar couplings (RDCs) and residual anisotropic chemical shifts (RACSs) are produced by the partial alignment of solution NMR samples. RDCs and RACSs yield high-resolution structural and dynamic information on the orientation of bonds and… read more here.
Keywords: solution nmr; residual dipolar; symmetry; molecular symmetry ... See more keywords
Effect of urea concentration on instant refolding of Nuclear Export Protein (NEP) from Influenza-A virus H1N1: A solution NMR based investigation.
Sign Up to like & getrecommendations! Published in 2020 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2020.10.146
Abstract: Nuclear-export-protein (NEP) plays multiple-functions during influenza virus replication-cycle and shows unique pattern of conserved residues, which altogether make NEP a potential target for developing novel anti-influenza drugs. However, the mechanistic structural biology of NEP has… read more here.
Keywords: solution nmr; nuclear export; protein nep; solution ... See more keywords
Two distinct conformers of SAG investigated by solution NMR and variable‐temperature experiments
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Pharmaceutical and Biomedical Analysis"
DOI: 10.1016/j.jpba.2018.12.019
Abstract: &NA; SAG (Smo agonist) is high specific agonist of Smo receptor, it is widely used as chemical probe to explore the therapeutic value of activating the Hh‐signaling pathway. In the present work, the coexistence of… read more here.
Keywords: solution nmr; distinct conformers; smo receptor; conformers sag ... See more keywords
AlphaFold Models of Small Proteins Rival the Accuracy of Solution NMR Structures
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2022.877000
Abstract: Recent advances in molecular modeling using deep learning have the potential to revolutionize the field of structural biology. In particular, AlphaFold has been observed to provide models of protein structures with accuracy rivaling medium-resolution X-ray… read more here.
Keywords: alphafold models; nmr structures; nmr; solution nmr ... See more keywords