Generation of reproductive transgenic pigs of a CRISPR‐Cas9‐based oncogene‐inducible system by somatic cell nuclear transfer
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Journal"
DOI: 10.1002/biot.202100434
Abstract: Alternative cancer models that are close to humans are required to create more valuable preclinical results during oncology studies. Here, a new onco‐pig model via developing a CRISPR‐Cas9‐based Conditional Polycistronic gene expression Cassette (CRI‐CPC) system… read more here.
Keywords: crispr cas9; system; cri cpc; cas9 based ... See more keywords
Use of mitochondrial sequencing to detect gene doping in horses via gene editing and somatic cell nuclear transfer.
Sign Up to like & getrecommendations! Published in 2022 at "Drug testing and analysis"
DOI: 10.1002/dta.3267
Abstract: Gene editing and subsequent cloning techniques offer great potential not only in genetic disease correction in domestic animals, but also in livestock production by enhancement of desirable traits. The existence of the technology, however, leaves… read more here.
Keywords: mitochondrial sequencing; nuclear transfer; cell nuclear; somatic cell ... See more keywords
Sperm‐derived RNAs improve the efficiency of somatic cell nuclear transfer (SCNT) through promoting R‐loop formation
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Reproduction and Development"
DOI: 10.1002/mrd.23627
Abstract: Mammalian sperm and oocytes are haploid cells that carry parental genetic and epigenetic information for their progeny. The cytoplasm of oocytes is also capable of reprograming somatic cells to establish totipotency through somatic cell nuclear… read more here.
Keywords: sperm derived; somatic cell; cell nuclear; sperm ... See more keywords
Optimized Protocol of Zebrafish Somatic Cell Nuclear Transfer (SCNT).
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9009-2_22
Abstract: Zebrafish (Danio rerio) is an established animal model to study developmental biology as well as a wide array of human diseases. Here we describe a protocol for somatic cell nuclear transfer (SCNT). This protocol can… read more here.
Keywords: somatic cell; cell nuclear; nuclear transfer; biology ... See more keywords
Episomal minicircles persist in periods of transcriptional inactivity and can be transmitted through somatic cell nuclear transfer into bovine embryos
Sign Up to like & getrecommendations! Published in 2019 at "Molecular Biology Reports"
DOI: 10.1007/s11033-019-04624-x
Abstract: Episomal plasmids based on a scaffold/matrix attachment region (S/MAR) are extrachromosomal DNA entities that replicate once per cell cycle and are stably maintained in cells or tissue. We generated minicircles, episomal plasmids devoid of bacterial… read more here.
Keywords: somatic cell; cell nuclear; bovine; episomal minicircles ... See more keywords
Comparison between direct and indirect immunofluorescence method for determination of somatic cell count
Sign Up to like & getrecommendations! Published in 2018 at "Chemical Papers"
DOI: 10.1007/s11696-018-0445-3
Abstract: A sensitive indirect immunofluorescence (IF) assay for bovine neutrophil and somatic cell counting was developed. The obtained indirect IF was compared with direct IF. The direct IF method uses a single-fluorophore-conjugated antibody directed against the target… read more here.
Keywords: somatic cell; indirect immunofluorescence; antibody; method ... See more keywords
Effect of Zinc Nanoparticles on Milk Yield, Milk Composition and Somatic Cell Count in Early-Lactating Barbari Does
Sign Up to like & getrecommendations! Published in 2019 at "Biological Trace Element Research"
DOI: 10.1007/s12011-019-01900-6
Abstract: Nano minerals have better bioavailability, efficient utilization, smaller dose rate, and stable interactions within the biological system. Efficacy of dietary supplementation of nano zinc particles on milk yield, milk composition, and somatic cell count was… read more here.
Keywords: milk; somatic cell; zinc; cell count ... See more keywords
Nac1 facilitates pluripotency gene activation for establishing somatic cell reprogramming.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2019.08.043
Abstract: Transcription factors play a central role in pluripotency transcription circuitry for establishing pluripotent reprogramming. Master transcription factors Oct4, Nanog, and Sox2 are known to form the core of the pluripotency transcription network. Other transcription factors… read more here.
Keywords: transcription; somatic cell; cell reprogramming; pluripotency ... See more keywords
Tracking and Predicting Human Somatic Cell Reprogramming Using Nuclear Characteristics.
Sign Up to like & getrecommendations! Published in 2019 at "Biophysical journal"
DOI: 10.1016/j.bpj.2019.10.014
Abstract: Reprogramming of human somatic cells to induced pluripotent stem cells (iPSCs) generates valuable resources for disease modeling, toxicology, cell therapy, and regenerative medicine. However, the reprogramming process can be stochastic and inefficient, creating many partially… read more here.
Keywords: somatic cell; human somatic; tracking predicting; predicting human ... See more keywords
Cloning of Macaque Monkeys by Somatic Cell Nuclear Transfer
Sign Up to like & getrecommendations! Published in 2018 at "Cell"
DOI: 10.1016/j.cell.2018.01.036
Abstract: (Cell 172, 881–887; February 8, 2018) In the above article, the raw data of RNA-sequencing, SNP, and STR were not initially uploaded into a public repository. These data have now been deposited and can be… read more here.
Keywords: monkeys somatic; somatic cell; cloning macaque; cell nuclear ... See more keywords
Robust preclinical evidence in somatic cell genome editing: a key driver of responsible and efficient therapeutic innovations.
Sign Up to like & getrecommendations! Published in 2021 at "Drug discovery today"
DOI: 10.1016/j.drudis.2021.06.007
Abstract: Somatic cell genome editing (SCGE) shows significant promising for therapeutic innovation. This study demonstrates that the majority of 46 preclinical SCGE studies discussed in reviews as particularly promising for clinical translation do not report on… read more here.
Keywords: genome editing; cell genome; research; somatic cell ... See more keywords