User‐Controlled 4D Biomaterial Degradation with Substrate‐Selective Sortase Transpeptidases for Single‐Cell Biology
Sign Up to like & getrecommendations! Published in 2023 at "Advanced Materials"
DOI: 10.1002/adma.202209904
Abstract: Stimuli‐responsive biomaterials show great promise for modeling disease dynamics ex vivo with spatiotemporal control over the cellular microenvironment. However, harvesting cells from such materials for downstream analysis without perturbing their state remains an outstanding challenge… read more here.
Keywords: cell; sortase; single cell; substrate ... See more keywords
Engineering sortase A by screening a second‐generation library using phage display
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Peptide Science"
DOI: 10.1002/psc.2980
Abstract: Sortase‐mediated ligation is one of the most commonly used chemo‐enzymatic techniques for the site‐specific modification of proteins. We have established a new library of sortase mutants for directed evolution of sortase substrate selectivity. Phage display… read more here.
Keywords: second generation; sortase; generation library; phage display ... See more keywords
Overcoming the Limitations of Sortase with Proximity-Based Sortase-Mediated Ligation (PBSL).
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9537-0_13
Abstract: S. aureus sortase A (SrtA), a calcium-dependent bacterial transpeptidase, is commonly used to site-specifically label proteins containing a LPXTG SrtA recognition motif with a wide array of chemical moieties. A major limitation of sortase-mediated labeling,… read more here.
Keywords: sortase; mediated ligation; proximity based; sortase mediated ... See more keywords
A comprehensive in silico analysis of sortase superfamily
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Microbiology"
DOI: 10.1007/s12275-019-8545-5
Abstract: Sortases are cysteine transpeptidases that assemble surface proteins and pili in their cell envelope. Encoded by all Gram-positive bacteria, few Gram-negative bacteria and archaea, sortases are currently divided into six classes (A-F). Due to the… read more here.
Keywords: comprehensive silico; class; analysis sortase; analysis ... See more keywords
Sortase A as a cross-linking enzyme in tissue engineering.
Sign Up to like & getrecommendations! Published in 2018 at "Acta biomaterialia"
DOI: 10.1016/j.actbio.2018.07.020
Abstract: The bacterial ligase Sortase A (SA) and its mutated variants have become increasingly popular over the last years for post-translational protein modifications due to their unparalleled specificity and efficiency. The aim of this work was… read more here.
Keywords: cross; sortase; cross linking; tissue engineering ... See more keywords
Sortase-Mediated Phage Decoration for Analytical Applications.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c02322
Abstract: Phage-borne peptides and antibody fragments isolated from phage display libraries have proven to be versatile and valuable reagents for immunoassay development. Due to the lack of convenient and mild-condition methods for the labeling of the… read more here.
Keywords: mediated phage; phage decoration; phage; sortase mediated ... See more keywords
Visualization and Quantification of Sortase Activity at the Single-Molecule Level via Transpeptidation-Directed Intramolecular Förster Resonance Energy Transfer.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.8b03716
Abstract: The sortase-catalyzed coupling reaction is an efficient strategy to incorporate chemically defined modifications into proteins of interest. Despite its widespread applications in protein chemistry, the conventional bulk fluorescence measurement is not sufficient to characterize sortase… read more here.
Keywords: single molecule; molecule level; sortase; activity ... See more keywords
Efficient Sortase-Mediated Ligation Using a Common C-Terminal Fusion Tag.
Sign Up to like & getrecommendations! Published in 2020 at "Bioconjugate chemistry"
DOI: 10.1021/acs.bioconjchem.0c00156
Abstract: Sortase-mediated ligation is a powerful method for generating site-specifically modified proteins. However, this process is limited by the inherent reversibility of the ligation reaction. To address this, here we report the continued development and optimization… read more here.
Keywords: mediated ligation; efficient sortase; ligation; sortase mediated ... See more keywords
Efficient Labeling of Native Human IgG by Proximity-Based Sortase-Mediated Isopeptide Ligation.
Sign Up to like & getrecommendations! Published in 2021 at "Bioconjugate chemistry"
DOI: 10.1021/acs.bioconjchem.1c00099
Abstract: Antibody-drug conjugates (ADCs) have demonstrated great therapeutic potential due to their ability to target the delivery of potent cytotoxins. However, the heterogeneous nature of conventional drug conjugation strategies can affect the safety, efficacy, and stability… read more here.
Keywords: igg; ligation; labeling native; efficient labeling ... See more keywords
Combined Application of Orthogonal Sortases and Depsipeptide Substrates for Dual Protein Labeling
Sign Up to like & getrecommendations! Published in 2022 at "Bioconjugate Chemistry"
DOI: 10.1021/acs.bioconjchem.2c00411
Abstract: Staphylococcus aureus sortase A is a transpeptidase that has been extensively exploited for site-specific modification of proteins and was originally used to attach a labeling reagent containing an LPXTG recognition sequence to a protein or… read more here.
Keywords: protein labeling; depsipeptide substrates; sortase; combined application ... See more keywords
Sortase-Mediated Site-Specific Conjugation and 89Zr-Radiolabeling of Designed Ankyrin Repeat Proteins for PET.
Sign Up to like & getrecommendations! Published in 2022 at "Molecular pharmaceutics"
DOI: 10.1021/acs.molpharmaceut.2c00136
Abstract: Designed ankyrin repeat proteins (DARPins) are genetically engineered proteins that exhibit high specificity and affinity toward specific targets. Here, the G3-DARPin, which binds the HER2/neu receptor, was site-specifically modified with enzymatic methods and 89Zr-radiolabeled for… read more here.
Keywords: designed ankyrin; sortase; 89zrdfo darpin; darpin ... See more keywords