Multiplex digital spatial profiling of proteins and RNA in fixed tissue
Sign Up to like & getrecommendations! Published in 2020 at "Nature Biotechnology"
DOI: 10.1038/s41587-020-0472-9
Abstract: Digital Spatial Profiling (DSP) is a method for highly multiplex spatial profiling of proteins or RNAs suitable for use on formalin-fixed, paraffin-embedded (FFPE) samples. The approach relies on (1) multiplexed readout of proteins or RNAs… read more here.
Keywords: digital spatial; proteins rna; rna fixed; profiling proteins ... See more keywords
305 Technical considerations for normalizing digital spatial profiling data with multiple within-patient samples
Sign Up to like & getrecommendations! Published in 2020 at "Journal for ImmunoTherapy of Cancer"
DOI: 10.1136/jitc-2020-sitc2020.0305
Abstract: Background NanoString’s GeoMx Digital Spatial Profiling (DSP) technology enables profiling of gene or protein expression from fresh or archival tissues. Specific regions of interest (ROIs) are identified via fluorescently labeled visualization markers. Within a given… read more here.
Keywords: digital spatial; considerations normalizing; expression; spatial profiling ... See more keywords
Spatial profiling reveals association between WNT pathway activation and T-cell exclusion in acquired resistance of synovial sarcoma to NY-ESO-1 transgenic T-cell therapy
Sign Up to like & getrecommendations! Published in 2022 at "Journal for Immunotherapy of Cancer"
DOI: 10.1136/jitc-2021-004190
Abstract: Background Genetically engineered T-cell immunotherapies for adoptive cell transfer (ACT) have emerged as a promising form of cancer treatment, but many of these patients develop recurrent disease. Furthermore, delineating mechanisms of resistance may be challenging… read more here.
Keywords: acquired resistance; cell; tumor; resistance ... See more keywords
Quantitative Spatial Profiling of Immune Populations in Pancreatic Ductal Adenocarcinoma Reveals Tumor Microenvironment Heterogeneity and Prognostic Biomarkers
Sign Up to like & getrecommendations! Published in 2022 at "Cancer Research"
DOI: 10.1158/0008-5472.can-22-1190
Abstract: Quantitative image analysis of PDAC specimens reveals intertumoral and intratumoral heterogeneity of immune populations and identifies spatial immune architectures that are significantly associated with disease prognosis. read more here.
Keywords: populations pancreatic; heterogeneity; immune populations; quantitative spatial ... See more keywords
Abstract 3810: Validation of novel high-plex protein spatial profiling quantitation based on NanoString's Digital Spatial Profiling (DSP) technology with quantitative fluorescence (QIF)
Sign Up to like & getrecommendations! Published in 2017 at "Cancer Research"
DOI: 10.1158/1538-7445.am2017-3810
Abstract: Background: Protein expression in formalin-fixed, paraffin-embedded (FFPE) tissue is routinely measured by Immunohistochemistry (IHC) on only one protein, or with quantitative fluorescence (QIF) on a handful of proteins on a single section. NanoString’s Digital Spatial… read more here.
Keywords: dsp technology; qif; tissue; dsp ... See more keywords
Abstract 4633: Quantitative spatial profiling of NSCLC subtypes across tumor stages using 6-plex multiplex imaging technology and AI-powered phenotyping analysis
Sign Up to like & getrecommendations! Published in 2023 at "Cancer Research"
DOI: 10.1158/1538-7445.am2023-4633
Abstract: Non-small cell lung cancer (NSCLC) is a complex disease with varying pathological subtypes including adenocarcinomas and squamous cell carcinomas. After diagnosis, tumor staging provides important information about the extent of cancer in the body and… read more here.
Keywords: nsclc subtypes; cell; imaging technology; analysis ... See more keywords
Digital Spatial Profiling of Individual Glomeruli From Patients With Anti-Neutrophil Cytoplasmic Autoantibody-Associated Glomerulonephritis
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2022.831253
Abstract: We previously showed that the rupture of Bowman’s capsule (BC) promotes the progression of crescentic glomerulonephritis by enhancing the entry of CD8+ T cells into the glomeruli. In the present study, we utilized digital spatial… read more here.
Keywords: glomeruli; neutrophil cytoplasmic; spatial profiling; digital spatial ... See more keywords
A review of spatial profiling technologies for characterizing the tumor microenvironment in immuno-oncology
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2022.996721
Abstract: Interpreting the mechanisms and principles that govern gene activity and how these genes work according to -their cellular distribution in organisms has profound implications for cancer research. The latest technological advancements, such as imaging-based approaches… read more here.
Keywords: oncology; tumor microenvironment; profiling technologies; spatial profiling ... See more keywords