Dynamic Organellar Maps for Spatial Proteomics
Sign Up to like & getrecommendations! Published in 2019 at "Current Protocols in Cell Biology"
DOI: 10.1002/cpcb.81
Abstract: Eukaryotic cells are highly compartmentalized and protein subcellular localization critically influences protein function. Identification of the subcellular localizations of proteins and their translocation events upon perturbation has mostly been confined to targeted studies or laborious… read more here.
Keywords: maps spatial; localization; spatial proteomics; biology ... See more keywords
MALDI-IHC-Guided In-Depth Spatial Proteomics: Targeted and Untargeted MSI Combined
Sign Up to like & getrecommendations! Published in 2023 at "Analytical Chemistry"
DOI: 10.1021/acs.analchem.2c04220
Abstract: Recently, a novel technology was published, utilizing the strengths of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) and immunohistochemistry (IHC), achieving highly multiplexed, targeted imaging of biomolecules in tissue. This new technique, called MALDI-IHC,… read more here.
Keywords: maldi ihc; spatial proteomics; ihc; msi ... See more keywords
Application of Machine Learning in Spatial Proteomics
Sign Up to like & getrecommendations! Published in 2022 at "Journal of chemical information and modeling"
DOI: 10.1021/acs.jcim.2c01161
Abstract: Spatial proteomics is an interdisciplinary field that investigates the localization and dynamics of proteins, and it has gained extensive attention in recent years, especially the subcellular proteomics. Numerous evidence indicate that the subcellular localization of… read more here.
Keywords: spatial proteomics; machine learning; application machine; proteomic data ... See more keywords
Combining LOPIT with differential ultracentrifugation for high-resolution spatial proteomics
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-018-08191-w
Abstract: The study of protein localisation has greatly benefited from high-throughput methods utilising cellular fractionation and proteomic profiling. Hyperplexed Localisation of Organelle Proteins by Isotope Tagging (hyperLOPIT) is a well-established method in this area. It achieves… read more here.
Keywords: protein; resolution; high resolution; localisation ... See more keywords
Spatial-proteomics reveals phospho-signaling dynamics at subcellular resolution
Sign Up to like & getrecommendations! Published in 2021 at "Nature Communications"
DOI: 10.1038/s41467-021-27398-y
Abstract: Dynamic change in subcellular localization of signaling proteins is a general concept that eukaryotic cells evolved for eliciting a coordinated response to stimuli. Mass spectrometry-based proteomics in combination with subcellular fractionation can provide comprehensive maps… read more here.
Keywords: phospho signaling; spatial proteomics; reveals phospho; proteomics reveals ... See more keywords
Preprint Highlight: Functional and spatial proteomics profiling reveals intra- and intercellular signaling crosstalk in colorectal cancer.
Sign Up to like & getrecommendations! Published in 2022 at "Molecular biology of the cell"
DOI: 10.1091/mbc.p22-10-1005
Abstract: Standard genomic and proteomic profiling methods for colorectal tumors do not always translate into reliable precision medicine outcomes. Tumor plasticity mechanisms can result in nongenetic responses to targeted therapies, calling for a functional approach using… read more here.
Keywords: spatial proteomics; preprint; colorectal cancer; signaling crosstalk ... See more keywords
Spatial Proteomics for the Molecular Characterization of Breast Cancer
Sign Up to like & getrecommendations! Published in 2023 at "Proteomes"
DOI: 10.3390/proteomes11020017
Abstract: Breast cancer (BC) is a major global health issue, affecting a significant proportion of the female population and contributing to high rates of mortality. One of the primary challenges in the treatment of BC is… read more here.
Keywords: spatial proteomics; breast cancer; molecular characterization; proteomics molecular ... See more keywords