Dynamic Organellar Maps for Spatial Proteomics
Sign Up to like & getrecommendations! Published in 2019 at "Current Protocols in Cell Biology"
DOI: 10.1002/cpcb.81
Abstract: Eukaryotic cells are highly compartmentalized and protein subcellular localization critically influences protein function. Identification of the subcellular localizations of proteins and their translocation events upon perturbation has mostly been confined to targeted studies or laborious… read more here.
Keywords: maps spatial; localization; spatial proteomics; biology ... See more keywords
MALDI-IHC-Guided In-Depth Spatial Proteomics: Targeted and Untargeted MSI Combined
Sign Up to like & getrecommendations! Published in 2023 at "Analytical Chemistry"
DOI: 10.1021/acs.analchem.2c04220
Abstract: Recently, a novel technology was published, utilizing the strengths of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) and immunohistochemistry (IHC), achieving highly multiplexed, targeted imaging of biomolecules in tissue. This new technique, called MALDI-IHC,… read more here.
Keywords: maldi ihc; spatial proteomics; ihc; msi ... See more keywords
Application of Machine Learning in Spatial Proteomics
Sign Up to like & getrecommendations! Published in 2022 at "Journal of chemical information and modeling"
DOI: 10.1021/acs.jcim.2c01161
Abstract: Spatial proteomics is an interdisciplinary field that investigates the localization and dynamics of proteins, and it has gained extensive attention in recent years, especially the subcellular proteomics. Numerous evidence indicate that the subcellular localization of… read more here.
Keywords: spatial proteomics; machine learning; application machine; proteomic data ... See more keywords
Combining LOPIT with differential ultracentrifugation for high-resolution spatial proteomics
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-018-08191-w
Abstract: The study of protein localisation has greatly benefited from high-throughput methods utilising cellular fractionation and proteomic profiling. Hyperplexed Localisation of Organelle Proteins by Isotope Tagging (hyperLOPIT) is a well-established method in this area. It achieves… read more here.
Keywords: protein; resolution; high resolution; localisation ... See more keywords
Spatial-proteomics reveals phospho-signaling dynamics at subcellular resolution
Sign Up to like & getrecommendations! Published in 2021 at "Nature Communications"
DOI: 10.1038/s41467-021-27398-y
Abstract: Dynamic change in subcellular localization of signaling proteins is a general concept that eukaryotic cells evolved for eliciting a coordinated response to stimuli. Mass spectrometry-based proteomics in combination with subcellular fractionation can provide comprehensive maps… read more here.
Keywords: phospho signaling; spatial proteomics; reveals phospho; proteomics reveals ... See more keywords
Preprint Highlight: Functional and spatial proteomics profiling reveals intra- and intercellular signaling crosstalk in colorectal cancer.
Sign Up to like & getrecommendations! Published in 2022 at "Molecular biology of the cell"
DOI: 10.1091/mbc.p22-10-1005
Abstract: Standard genomic and proteomic profiling methods for colorectal tumors do not always translate into reliable precision medicine outcomes. Tumor plasticity mechanisms can result in nongenetic responses to targeted therapies, calling for a functional approach using… read more here.
Keywords: spatial proteomics; preprint; colorectal cancer; signaling crosstalk ... See more keywords
Statistical analysis of multiple regions-of-interest in multiplexed spatial proteomics data
Sign Up to like & getrecommendations! Published in 2024 at "Briefings in Bioinformatics"
DOI: 10.1093/bib/bbae522
Abstract: Abstract Multiplexed spatial proteomics reveals the spatial organization of cells in tumors, which is associated with important clinical outcomes such as survival and treatment response. This spatial organization is often summarized using spatial summary statistics,… read more here.
Keywords: spatial proteomics; usepackage; multiplexed spatial; statistical analysis ... See more keywords
Abstract 2762: Spatial proteomics of lung adenocarcinoma mouse models with divergent propensities for metastasis
Sign Up to like & getrecommendations! Published in 2024 at "Cancer Research"
DOI: 10.1158/1538-7445.am2024-2762
Abstract: Introduction: Lung cancer is the leading cause of cancer deaths worldwide with most deaths attributed to metastasis. Metastases in existing mouse models of lung adenocarcinoma (LUAD) is sporadic and often requires several months of aging.… read more here.
Keywords: cancer; spatial proteomics; mouse models; lung adenocarcinoma ... See more keywords
Abstract 6310: Bridging spatial proteomics and classical histopathology: advanced spatial analysis through H&E-enhanced multiplexed immunoprofiling
Sign Up to like & getrecommendations! Published in 2025 at "Cancer Research"
DOI: 10.1158/1538-7445.am2025-6310
Abstract: Multiplex immunofluorescence (mIF) is increasingly adopted in translational research due to its ability to provide rich spatial and molecular information. Nevertheless, H&E staining remains the gold standard for clinical diagnosis, revealing essential histological context and… read more here.
Keywords: mif; spatial proteomics; analysis; histopathology ... See more keywords
Spatial proteomics and transcriptomics reveal early immune cell organization in pancreatic intraepithelial neoplasia
Sign Up to like & getrecommendations! Published in 2025 at "JCI Insight"
DOI: 10.1172/jci.insight.191595
Abstract: Pancreatic ductal adenocarcinoma (PDAC) has a poor survival rate due to late detection. PDAC arises from precursor microscopic lesions, termed pancreatic intraepithelial neoplasia (PanIN), that develop at least a decade before overt disease; this provides… read more here.
Keywords: panin pdac; spatial proteomics; pancreatic intraepithelial; intraepithelial neoplasia ... See more keywords
Distinct morphological features of malignant cells in large B-cell lymphoma identified by soft-segmentation in imaging-based spatial proteomics
Sign Up to like & getrecommendations! Published in 2025 at "Blood"
DOI: 10.1182/blood-2025-4342
Abstract: Background: Mature B-cell neoplasms display densely packed and morphologically heterogeneous cells, often complicating tissue-level analysis. Recent studies have revealed the complexity of the tumor microenvironment (TME) in these malignancies (Roider, Nature Cell Biology 2024; Colombo,… read more here.
Keywords: spatial proteomics; segmentation; cluster; cell ... See more keywords