Surface Ligand Engineering Ruthenium Nanozyme Superior to Horseradish Peroxidase for Enhanced Immunoassay.
Sign Up to like & getrecommendations! Published in 2023 at "Advanced materials"
DOI: 10.1002/adma.202300387
Abstract: Nanozymes have great potential to be used as an alternative to natural enzymes in a variety of fields. However, low catalytic activity compared with natural enzymes limits their practical use. It is still challenging to… read more here.
Keywords: horseradish peroxidase; surface ligand; specific activity; activity ... See more keywords
Effects of the substituted amino acid residues on the thermal properties of monomeric isocitrate dehydrogenases from a psychrophilic bacterium, Psychromonas marina, and a mesophilic bacterium, Azotobacter vinelandii
Sign Up to like & getrecommendations! Published in 2019 at "Extremophiles"
DOI: 10.1007/s00792-019-01137-0
Abstract: A cold-adapted monomeric isocitrate dehydrogenase from a psychrophilic bacterium, Psychromonas marina (PmIDH), showed a high degree of amino acid sequential identity (64%) to a mesophilic one from a mesophilic bacterium, Azotobacter vinelandii (AvIDH). In this… read more here.
Keywords: bacterium; amino acid; specific activity; thermostability activity ... See more keywords
Zymographic approach to determine the intrinsic enzyme specific activity of diamine oxidase in presence of interfering enzymes.
Sign Up to like & getrecommendations! Published in 2017 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2017.04.006
Abstract: The purpose of this investigation was to elaborate a fast zymographic assay of oxidase enzymes in the presence of interfering enzymes as catalase (which disturbs current dosages based on H2O2 detection). This method also allows… read more here.
Keywords: oxidase; presence; dao; specific activity ... See more keywords
Dynamic response analysis of corrosion products activity under steady state operation and Mechanical Shim based power-maneuvering transients in AP-1000
Sign Up to like & getrecommendations! Published in 2018 at "Annals of Nuclear Energy"
DOI: 10.1016/j.anucene.2018.01.009
Abstract: Abstract The advanced Mechanical Shim (MSHIM) core control strategy is a flexible power-maneuvering feature of AP-1000 reactor. This is vulnerable for variation in Activated Corrosion Products (ACPs) buildup while providing frequent power maneuver opportunities. It… read more here.
Keywords: power; specific activity; operation; power maneuvering ... See more keywords
Improving the specific activity and thermo-stability of alkaline pectate lyase from Bacillus subtilis 168 for bioscouring
Sign Up to like & getrecommendations! Published in 2018 at "Biochemical Engineering Journal"
DOI: 10.1016/j.bej.2017.11.001
Abstract: Abstract Biocatalysts requires enzymes with high activity and stability under process conditions for efficient application. Several protein improvement strategies were used to improve pectate lyase PEL168 from Bacillus subtilis. Initially, a rationally designed mutant V132F… read more here.
Keywords: specific activity; k47d v132f; pectate lyase; stability ... See more keywords
Overexpression and enhanced specific activity of aldoketo reductases (AKR1B1 & AKR1B10) in human breast cancers.
Sign Up to like & getrecommendations! Published in 2017 at "Breast"
DOI: 10.1016/j.breast.2016.11.003
Abstract: The incidence of breast cancer in India is on the rise and is rapidly becoming the primary cancer in Indian women. The aldoketo reductase (AKR) family has more than 190 proteins including aldose reductase (AKR1B1)… read more here.
Keywords: breast; specific activity; akr1b1 akr1b10; cancer ... See more keywords
A user-specific activity pattern generation framework for evidence-based ABW planning
Sign Up to like & getrecommendations! Published in 2020 at "Building and Environment"
DOI: 10.1016/j.buildenv.2020.107519
Abstract: Abstract To plan successful activity-based workplaces (ABW), architects need to clearly understand user-specific activity patterns through the accurate recognition of user activity. Because user activity is closely associated with space, equipment, and users, such diverse… read more here.
Keywords: activity pattern; space; user specific; specific activity ... See more keywords
Role of N-glycosylation on the specific activity of a Coprinopsis cinerea laccase Lcc9 expressed in Pichia pastoris.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of bioscience and bioengineering"
DOI: 10.1016/j.jbiosc.2019.05.004
Abstract: Laccase Lcc9 from Coprinopsis cinerea heterologously expressed in Pichia pastoris (rLcc9) displayed different molecular weight and specific activity from the native laccase (nLcc9). Glycosylation may play a role in regulating the Lcc9 specific activity. To… read more here.
Keywords: laccase lcc9; glycosylation; specific activity; coprinopsis cinerea ... See more keywords
Directed modification of l-LcLDH1, an l-lactate dehydrogenase from Lactobacillus casei, to improve its specific activity and catalytic efficiency towards phenylpyruvic acid.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of biotechnology"
DOI: 10.1016/j.jbiotec.2018.05.011
Abstract: To improve the specific activity and catalytic efficiency of l-LcLDH1, an NADH-dependent allosteric l-lactate dehydrogenase from L. casei, towards phenylpyruvic acid (PPA), its directed modification was conducted based on the semi-rational design. The three variant… read more here.
Keywords: improve specific; activity catalytic; specific activity; catalytic efficiency ... See more keywords
Recyclable Thermoresponsive Polymer-β-Glucosidase Conjugate with Intact Hydrolysis Activity.
Sign Up to like & getrecommendations! Published in 2018 at "Biomacromolecules"
DOI: 10.1021/acs.biomac.8b00258
Abstract: β-Glucosidase (BG) catalyzes the hydrolysis of cellobiose to glucose and is a rate-limiting enzyme in the conversion of lignocellulosic biomass to sugars toward biofuels. Since the cost of enzyme is a major contributor to biofuel… read more here.
Keywords: conjugate; polymer; hydrolysis; specific activity ... See more keywords
Imaging the Site-Specific Activity and Kinetics on a Single Nanomaterial by Microchamber Array
Sign Up to like & getrecommendations! Published in 2017 at "ACS Catalysis"
DOI: 10.1021/acscatal.6b03518
Abstract: One-dimensional (1D) and two-dimensional (2D) nanomaterials usually show diverse catalytic activities at different locations. For a particular location, the local reaction activity could be too fast to be measured by super-resolution microscopy (SRM). To solve… read more here.
Keywords: array; research; site specific; specific activity ... See more keywords