Isolation of Antigen-Specific, Antibody-Secreting Cells Using a Chip-Based Immunospot Array.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8958-4_6
Abstract: Antigen-specific monoclonal antibodies are useful tools to detect very small amounts of antigenic materials and are applicable for antibody therapeutics. To produce mouse monoclonal antibodies, a hybridoma between B lymphocytes and myeloma cells is used… read more here.
Keywords: antibody secreting; monoclonal antibodies; specific antibody; antigen specific ... See more keywords
P029 A successful case of stem cell transplant after desensitization of a strong donor-specific antibody
Sign Up to like & getrecommendations! Published in 2017 at "Human Immunology"
DOI: 10.1016/j.humimm.2017.06.089
Abstract: A 71 year old female was initially diagnosed with myelodysplastic syndrome (del 5q) which progressed to AML one year later. Patient had pancytopenia, experienced bruising, and needed blood transfusions. Allogeneic stem cell transplant was recommended and… read more here.
Keywords: stem cell; specific antibody; cell transplant; donor specific ... See more keywords
P080 Case report, BANFF III rejection with IGM-donor specific antibody (DSA)
Sign Up to like & getrecommendations! Published in 2017 at "Human Immunology"
DOI: 10.1016/j.humimm.2017.06.140
Abstract: Aim Testing for IgM anti-HLA antibodies is not routinely tested in the majority of the HLA laboratories, despite the harm that they might cause. We report here a case of 69 years old male with End… read more here.
Keywords: specific antibody; rejection; case; igm ... See more keywords
P040 Post-transplant De-novo donor specific antibody to HLA-B8, of unknown clinical significance
Sign Up to like & getrecommendations! Published in 2018 at "Human Immunology"
DOI: 10.1016/j.humimm.2018.07.098
Abstract: Clinical History: A 62 year-old male with chronic glomerulonephritis underwent kidney transplant from a donation after cardiac death donor in Jan 2017. Cold ischemia time was 8 h. The recipient had no donor specific antibody detected in… read more here.
Keywords: donor; specific antibody; antibody; post transplant ... See more keywords
Polyvalent immunoglobulin binding is an obstacle to accurate measurement of specific antibodies with ELISA despite inclusion of blocking agents.
Sign Up to like & getrecommendations! Published in 2017 at "International immunopharmacology"
DOI: 10.1016/j.intimp.2017.09.016
Abstract: Specific antibody concentrations are frequently measured in serum (and plasma and intravenous immunoglobulin) samples by enzyme-linked immunosorbent assay (ELISA). The standard negative control involves incubation of buffer alone on antigen-coated wells. The immunoreactivity that develops… read more here.
Keywords: immunoglobulin; blocking agents; specific antibody; antibody ... See more keywords
Sequence and functional characterization of a public HIV-specific antibody clonotype
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DOI: 10.1016/j.isci.2021.103564
Abstract: Summary Public antibody clonotypes shared among multiple individuals have been identified for several pathogens. However, little is known about the determinants of antibody “publicness”. Here, we characterize the sequence and functional properties of antibodies from… read more here.
Keywords: sequence; specific antibody; antibody clonotype; antibody ... See more keywords
Regulation of T- and B-cell interactions determines the clinical phenotype associated with donor-specific antibodies.
Sign Up to like & getrecommendations! Published in 2022 at "Kidney international"
DOI: 10.1016/j.kint.2022.02.020
Abstract: The cellular mechanisms that regulate donor-specific antibody formation and antibody-mediated rejection remain unknown. In this issue, Louis et al. report that specific T-regulatory cell and B-regulatory transitional cell subsets are concomitantly diminished in patients with donor-specific… read more here.
Keywords: donor specific; cell; antibody; specific antibody ... See more keywords
Proximity-Induced Site-Specific Antibody Conjugation.
Sign Up to like & getrecommendations! Published in 2018 at "Bioconjugate chemistry"
DOI: 10.1021/acs.bioconjchem.8b00680
Abstract: Site-specific antibody conjugates with a well-defined structure and superb therapeutic index are of great interest for basic research, disease diagnostics, and therapy. Here, we develop a novel proximity-induced antibody conjugation strategy enabling site-specific covalent bond… read more here.
Keywords: site specific; specific antibody; site; conjugation ... See more keywords
SARS-CoV-2 specific antibody and neutralization assays reveal the wide range of the humoral immune response to virus
Sign Up to like & getrecommendations! Published in 2021 at "Communications Biology"
DOI: 10.1038/s42003-021-01649-6
Abstract: Development of antibody protection during SARS-CoV-2 infection is a pressing question for public health and for vaccine development. We developed highly sensitive SARS-CoV-2-specific antibody and neutralization assays. SARS-CoV-2 Spike protein or Nucleocapsid protein specific IgG… read more here.
Keywords: antibody neutralization; specific antibody; antibody; sars cov ... See more keywords
New linker structures applied in glycosite-specific antibody drug conjugates
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DOI: 10.1039/c9qo00646j
Abstract: Two new linkers employed in glycosite-specific antibody–drug conjugates demonstrate efficient conjugation, enhanced stability, and fluorescence properties. read more here.
Keywords: drug conjugates; antibody drug; specific antibody; new linker ... See more keywords
Continued Virus-Specific Antibody-Secreting Cell Production, Avidity Maturation and B Cell Evolution in Patients Hospitalized with COVID-19.
Sign Up to like & getrecommendations! Published in 2022 at "Viral immunology"
DOI: 10.1089/vim.2021.0191
Abstract: Understanding the development and sustainability of the virus-specific protective immune response to infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) remains incomplete with respect to the appearance and disappearance of virus-specific antibody-secreting cells (ASCs) in… read more here.
Keywords: cell; specific antibody; virus specific; igg ... See more keywords