An in vitro site-specific cleavage assay of CRISPR-Cas9 using a personal glucose meter.
Sign Up to like & getrecommendations! Published in 2020 at "Chemical communications"
DOI: 10.1039/d0cc03505j
Abstract: A sensitive and cost-effective assay based on a personal glucose meter was developed to detect the in vitro site-specific cleavage efficiency of CRISPR-Cas9. read more here.
Keywords: personal glucose; site specific; crispr cas9; vitro site ... See more keywords
Homologous VapC Toxins Inhibit Translation and Cell Growth by Sequence-Specific Cleavage of tRNAfMet
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Bacteriology"
DOI: 10.1128/jb.00582-17
Abstract: ABSTRACT Type II toxin-antitoxin (TA) systems play a critical role in the establishment and maintenance of bacterial dormancy. They are composed of a protein toxin and its cognate protein antitoxin. They function to regulate growth… read more here.
Keywords: dormancy; vapc toxins; toxin; specific cleavage ... See more keywords