A chemoenzymatic approach enables the site‐specific conjugation of recombinant proteins
Sign Up to like & getrecommendations! Published in 2019 at "ELECTROPHORESIS"
DOI: 10.1002/elps.201800528
Abstract: Many biotechniques including protein microarray, drug screening, biosensors rely on the immobilization of recombinant proteins on the solid supports. It is well known that random orientation of the immobilized proteins could impair their biologic functions.… read more here.
Keywords: site specific; site; specific conjugation; recombinant proteins ... See more keywords
Affinity-Based Methods for Site-Specific Conjugation of Antibodies
Sign Up to like & getrecommendations! Published in 2021 at "Bioconjugate Chemistry"
DOI: 10.1021/acs.bioconjchem.1c00313
Abstract: Conjugation of various reagents to antibodies has long been an elegant way to combine the superior binding features of the antibody with other desired but non-natural functions. Applications range from labels for detection in different… read more here.
Keywords: conjugation antibodies; affinity; site specific; site ... See more keywords
Protein-Grafted Polymers Prepared Through a Site-Specific Conjugation by Microbial Transglutaminase for an Immunosorbent Assay.
Sign Up to like & getrecommendations! Published in 2017 at "Biomacromolecules"
DOI: 10.1021/acs.biomac.6b01538
Abstract: Protein-polymer conjugates have been developed in many fields. Most hybrids are composed of one protein attached to one or several polymer chains. The other form of hybrid involves the construction of multiple proteins on one… read more here.
Keywords: protein; site specific; polymer; specific conjugation ... See more keywords
Tag-free, specific conjugation of glycosylated IgG1 antibodies using microbial transglutaminase
Sign Up to like & getrecommendations! Published in 2022 at "RSC Advances"
DOI: 10.1039/d2ra05630e
Abstract: We present an efficient approach for tag-free, site-specific conjugation of a fully glycosylated antibody using microbial transglutaminase (mTG). We created variants of trastuzumab where a single surface-exposed residue of the human crystallizable fragment had been… read more here.
Keywords: using microbial; tag free; specific conjugation; microbial transglutaminase ... See more keywords