An Improved Method for Identifying Specific DNA-Protein-Binding Sites In Vitro
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Biotechnology"
DOI: 10.1007/s12033-017-9993-y
Abstract: Binding of proteins to specific DNA sequences is essential for a variety of cellular processes such as DNA replication, transcription and responses to external stimuli. Chromatin immunoprecipitation is widely used for determining intracellular DNA fragments… read more here.
Keywords: protein; protein binding; dna; method identifying ... See more keywords
Systematic Design of Adenosine Analogs as Inhibitors of a Clostridioides difficile-Specific DNA Adenine Methyltransferase Required for Normal Sporulation and Persistence
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Medicinal Chemistry"
DOI: 10.1021/acs.jmedchem.2c01789
Abstract: Antivirulence agents targeting endospore-transmitted Clostridioides difficile infections are urgently needed. C. difficile-specific DNA adenine methyltransferase (CamA) is required for efficient sporulation and affects persistence in the colon. The active site of CamA is conserved and… read more here.
Keywords: dna; clostridioides difficile; specific dna; difficile specific ... See more keywords
Comparative Study of Adenosine Analogs as Inhibitors of Protein Arginine Methyltransferases and a Clostridioides difficile-Specific DNA Adenine Methyltransferase
Sign Up to like & getrecommendations! Published in 2023 at "ACS Chemical Biology"
DOI: 10.1021/acschembio.3c00035
Abstract: S-Adenosyl-l-methionine (SAM) analogs are adaptable tools for studying and therapeutically inhibiting SAM-dependent methyltransferases (MTases). Some MTases play significant roles in host–pathogen interactions, one of which is Clostridioides difficile-specific DNA adenine MTase (CamA). CamA is needed… read more here.
Keywords: dna; clostridioides difficile; specific dna; difficile specific ... See more keywords
Translocation of Specific DNA Nanocarrier through an Ultrasmall Nanopipette: Toward Single-Protein-Molecule Detection with Superior Signal-to-Noise Ratio.
Sign Up to like & getrecommendations! Published in 2022 at "ACS nano"
DOI: 10.1021/acsnano.2c06303
Abstract: The use of functional DNA nanostructures as carriers to ship proteins through solid-state nanopores has recently seen substantial growth in single-protein-molecule detection (SPMD), driven by the potential of this methodology and implementations that it may… read more here.
Keywords: single protein; dna; protein molecule; detection ... See more keywords
Structurally Specific Z-DNA Proteolysis Targeting Chimera Enables Targeted Degradation of Adenosine Deaminase Acting on RNA 1.
Sign Up to like & getrecommendations! Published in 2024 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.3c13646
Abstract: Given the prevalent advancements in DNA- and RNA-based PROTACs, there remains a significant need for the exploration and expansion of more specific DNA-based tools, thus broadening the scope and repertoire of DNA-based PROTACs. Unlike conventional… read more here.
Keywords: degradation; specific dna; protacs; form dna ... See more keywords
Loss of N-6 adenine-specific DNA methyltransferase 1 leads to meiotic prophase abnormalities and male sub-fertility in mice.
Sign Up to like & getrecommendations! Published in 2025 at "Biology of reproduction"
DOI: 10.1093/biolre/ioaf052
Abstract: Mammalian sexual reproduction critically relies on the generation of haploid gametes following a specialized cell division process known as meiosis. Here, we demonstrate that N-6 Adenine-Specific DNA methyltransferase 1 (N6AMT1) plays a crucial role in… read more here.
Keywords: adenine specific; fertility mice; specific dna; dna methyltransferase ... See more keywords
Identification of Vibrio alginolyticus virulent strain‐specific DNA regions by suppression subtractive hybridization and PCR
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Applied Microbiology"
DOI: 10.1111/jam.14739
Abstract: Vibrio alginolyticus was frequently isolated from diseased farmed fish in the coaster waters of Hainan Island over the past two decades. In this study, we attempted to identify candidates of virulent strain‐specific DNA regions for… read more here.
Keywords: dna regions; virulent strain; specific dna; strain specific ... See more keywords
Structure-specific DNA recombination sites: Design, validation, and machine learning–based refinement
Sign Up to like & getrecommendations! Published in 2020 at "Science Advances"
DOI: 10.1126/sciadv.aay2922
Abstract: Synthetic attC sites can be embedded into virtually any sequence and constitute a structure-specific DNA recombination system. Recombination systems are widely used as bioengineering tools, but their sites have to be highly similar to a… read more here.
Keywords: dna; structure specific; specific dna; recombination ... See more keywords
Gene domain-specific DNA methylation episignatures highlight distinct molecular entities of ADNP syndrome
Sign Up to like & getrecommendations! Published in 2019 at "Clinical Epigenetics"
DOI: 10.1186/s13148-019-0658-5
Abstract: BackgroundADNP syndrome is a rare Mendelian disorder characterized by global developmental delay, intellectual disability, and autism. It is caused by truncating mutations in ADNP, which is involved in chromatin regulation. We hypothesized that the disruption… read more here.
Keywords: methylation episignatures; dna; specific dna; adnp syndrome ... See more keywords
Full-length human cytomegalovirus terminase pUL89 adopts a two-domain structure specific for DNA packaging
Sign Up to like & getrecommendations! Published in 2019 at "PLoS Pathogens"
DOI: 10.1371/journal.ppat.1008175
Abstract: A key step in replication of human cytomegalovirus (HCMV) in the host cell is the generation and packaging of unit-length genomes into preformed capsids. The enzymes involved in this process are the terminases. The HCMV… read more here.
Keywords: terminase; dna; specific dna; human cytomegalovirus ... See more keywords
The transformational impact of site-specific DNA modifiers on biomedicine and agriculture
Sign Up to like & getrecommendations! Published in 2018 at "Animal Reproduction"
DOI: 10.21451/1984-3143-ar2018-0065
Abstract: The development of genetically modified livestock has been dependent on incremental technological advances such as embryo transfer, homologous recombination, and somatic cell nuclear transfer (SCNT). This development rate has increased exponentially with the advent of… read more here.
Keywords: transformational impact; site specific; specific dna; impact ... See more keywords