Generation of a host cell line containing a MAR‐rich landing pad for site‐specific integration and expression of transgenes
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Progress"
DOI: 10.1002/btpr.3254
Abstract: In recent years, targeted gene integration (TI) has been introduced as a strategy for the generation of recombinant mammalian cell lines for the production of biotherapeutics. Besides reducing the immense heterogeneity within a pool of… read more here.
Keywords: site specific; cell; host cell; specific integration ... See more keywords
A system for site-specific integration of transgenes in mammalian cells
Sign Up to like & getrecommendations! Published in 2019 at "PLoS ONE"
DOI: 10.1371/journal.pone.0219842
Abstract: Mammalian cell expression systems are the most commonly used platforms for producing biotherapeutic proteins. However, development of recombinant mammalian cell lines is often hindered by the unstable and variable transgene expression associated with random integration.… read more here.
Keywords: system; integration; site specific; cell ... See more keywords