A species-specific multiplexed PCR amplicon assay for distinguishing between Metarhizium anisopliae, M. brunneum, M. pingshaense and M. robertsii.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of invertebrate pathology"
DOI: 10.1016/j.jip.2019.01.002
Abstract: The fungal species Metarhizium pingshaense, M. anisopliae, M. robertsii, and M. brunneum, a monophyletic group informally referred to as the PARB species complex, are well known facultative entomopathogens, including many commercialized strains used for biological… read more here.
Keywords: pcr; anisopliae; species specific; specific multiplexed ... See more keywords
Highly specific multiplexed RNA imaging in tissues with split-FISH
Sign Up to like & getrecommendations! Published in 2020 at "Nature Methods"
DOI: 10.1038/s41592-020-0858-0
Abstract: We present split-FISH, a multiplexed fluorescence in situ hybridization method that leverages a split-probe design to achieve enhanced specificity. Split-FISH reduces off-target background fluorescence, decreases false positives and enables accurate RNA profiling in uncleared tissues.… read more here.
Keywords: multiplexed rna; highly specific; tissues split; specific multiplexed ... See more keywords