Articles with "specific pcr" as a keyword



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Application of multiplex methylated‐specific PCR with capillary electrophoresis to explore prognostic value of TSGs hypermethylation for hepatocellular carcinoma

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Published in 2018 at "Journal of Clinical Laboratory Analysis"

DOI: 10.1002/jcla.22430

Abstract: Hepatocellular carcinoma (HCC) is a malignant tumor that severely threatens human health. To date, early detection for HCC patients is particularly significant due to their poor survival rates even after liver resection. read more here.

Keywords: hepatocellular carcinoma; specific pcr; carcinoma; application multiplex ... See more keywords
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Development of a Species-Specific PCR Assay for Correct Labeling of Eight Squid Species from Loliginidae and Ommastrephidae Families (Mollusca: Cephalopoda)

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Published in 2018 at "Food Analytical Methods"

DOI: 10.1007/s12161-018-1287-x

Abstract: Squid is an important seafood resource in Asian and European countries. Due to the different nutritional and market values of various squid species, substitutions between species often occur for higher profits. In this study, a… read more here.

Keywords: specific pcr; eight squid; pcr assay; squid species ... See more keywords
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Identification of S genotypes in loquat (Eriobotrya japonica Lindl.) based on allele specific PCR

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Published in 2017 at "Scientia Horticulturae"

DOI: 10.1016/j.scienta.2017.07.050

Abstract: Abstract Loquat (Eriobotrya japonica Lindl) is an evergreen fruit tree and belongs to Maloideae in the Rosaceae, which carries the RNase-dependent gametophytic self-incompatibility system (GSI). Use of appropriate pollinator trees is essential for increasing fruit… read more here.

Keywords: specific pcr; loquat eriobotrya; eriobotrya japonica; allele specific ... See more keywords
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Development and application of an allele-specific PCR assay for detecting T409C mutation of cyp51 gene linked with tebuconazole resistance in Villosiclava virens (rice false smut)

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Published in 2017 at "Canadian Journal of Plant Pathology"

DOI: 10.1080/07060661.2017.1356873

Abstract: Abstract Villosiclava virens is an ascomycete fungus (anamorph: Ustilaginoidea virens) recognized as one of the most economically important pathogens on rice in China that causes false smut disease. In our study, the V. virens cyp51… read more here.

Keywords: specific pcr; t409c mutation; pcr assay; villosiclava virens ... See more keywords

Pathovar-Specific PCR Method for Detection and Identification of Xanthomonas translucens pv. undulosa.

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Published in 2023 at "Plant disease"

DOI: 10.1094/pdis-11-22-2677-sr

Abstract: Bacterial leaf streak disease caused by Xanthomonas translucens pv. undulosa is an economically important disease threatening wheat and barley crops around the globe. So far, specific PCR-based detection and identification tests for X. translucens pathovars… read more here.

Keywords: translucens undulosa; xanthomonas translucens; detection identification; detection ... See more keywords
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Design of SARS-CoV-2 Variant-Specific PCR Assays Considering Regional and Temporal Characteristics

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Published in 2022 at "Applied and Environmental Microbiology"

DOI: 10.1128/aem.02289-21

Abstract: Monitoring the introduction and prevalence of variants of concern (VOCs) and variants of interest (VOIs) in a community can help the local authorities make informed public health decisions. PCR assays can be designed to keep… read more here.

Keywords: variant specific; pcr; pcr assays; sars cov ... See more keywords
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Detection of Antimalarial Resistance-Associated Mutations in Plasmodium falciparum via a Platform of Allele-Specific PCR Combined with a Gold Nanoparticle-Based Lateral Flow Assay

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Published in 2022 at "Microbiology Spectrum"

DOI: 10.1128/spectrum.02535-22

Abstract: Rapid detection of single nucleotide polymorphisms (SNPs) is essential for malaria treatment. Based on the techniques of allele-specific PCR (AS-PCR) and lateral flow assay (LFA), an accurate and powerful platform for SNP detection of pfmdr1… read more here.

Keywords: allele specific; lateral flow; detection; resistance ... See more keywords
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A Rapid and Specific Genotyping Platform for Plasmodium falciparum Chloroquine Resistance via Allele-Specific PCR with a Lateral Flow Assay

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Published in 2022 at "Microbiology Spectrum"

DOI: 10.1128/spectrum.02719-21

Abstract: Accurate recognition of the mutation and genotype of genes are essential for the treatment of infectious diseases and genetic diseases. Based on the techniques of allele-specific PCR (AS-PCR) and a lateral flow assay (LFA), a… read more here.

Keywords: flow assay; lateral flow; platform; allele specific ... See more keywords
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Optimization of allele specific PCR (AS-PCR) for the early detection of FLT3 (D835Y) mutation in Acute Myeloid Leukemia patients at Tabuk, Saudi Arabia.

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Published in 2017 at "International Journal of Approximate Reasoning"

DOI: 10.21474/ijar01/2819

Abstract: Abu-Duhier FM 1 , Rashid Mir 1 , Jamsheed Javid 1 , Fawzia Sharaf 2 and Nabil Burrow 2 . 1. Prince Fahd Bin Sultan Research chair, Department of Medical Laboratory Technology, Faculty of Applied… read more here.

Keywords: specific pcr; tabuk saudi; optimization allele; saudi arabia ... See more keywords
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Emerging Invasive Group A Streptococcus M1UK Lineage Detected by Allele-Specific PCR, England, 2020

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Published in 2023 at "Emerging Infectious Diseases"

DOI: 10.3201/eid2905.221887

Abstract: Increasing reports of invasive Streptococcus pyogenes infections mandate surveillance for toxigenic lineage M1UK. An allele-specific PCR was developed to distinguish M1UK from other emm1 strains. The M1UK lineage represented 91% of invasive emm1 isolates in… read more here.

Keywords: m1uk lineage; allele specific; england 2020; specific pcr ... See more keywords
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RAPID LOW-COST DETECTION OF TYPE 2CALR MUTATION BY ALLELE-SPECIFIC RT-PCR FOR DIAGNOSIS OF MYELOPROLIFERATIVE NEOPLASMS.

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Published in 2022 at "Experimental oncology"

DOI: 10.32471/exp-oncology.2312-8852.vol-44-no-1.17329

Abstract: BACKGROUND Approximately 15% to 24% of essential thrombocythemia (ET) and 25-35% of primary myelofibrosis cases carry a mutation in the calreticulin (CALR) gene. Sanger sequencing, qPCR, high resolution melt or targeted next generation sequencing usually… read more here.

Keywords: calr gene; allele specific; low cost; mutation ... See more keywords