Identification of diagnostic kompetitive allele‐specific PCR markers for selection of crown rust resistance in oats
Sign Up to like & getrecommendations! Published in 2025 at "Crop Science"
DOI: 10.1002/csc2.70076
Abstract: Crown rust disease, caused by Puccinia coronata f. sp. avenae, poses a significant threat to global oat (Avena sativa L.) production. Molecular markers are essential to assist in the integration of multiple resistance genes into… read more here.
Keywords: resistance; oat; allele specific; kompetitive allele ... See more keywords
Application of multiplex methylated‐specific PCR with capillary electrophoresis to explore prognostic value of TSGs hypermethylation for hepatocellular carcinoma
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Clinical Laboratory Analysis"
DOI: 10.1002/jcla.22430
Abstract: Hepatocellular carcinoma (HCC) is a malignant tumor that severely threatens human health. To date, early detection for HCC patients is particularly significant due to their poor survival rates even after liver resection. read more here.
Keywords: hepatocellular carcinoma; specific pcr; carcinoma; application multiplex ... See more keywords
Development of a Species-Specific PCR Assay for Correct Labeling of Eight Squid Species from Loliginidae and Ommastrephidae Families (Mollusca: Cephalopoda)
Sign Up to like & getrecommendations! Published in 2018 at "Food Analytical Methods"
DOI: 10.1007/s12161-018-1287-x
Abstract: Squid is an important seafood resource in Asian and European countries. Due to the different nutritional and market values of various squid species, substitutions between species often occur for higher profits. In this study, a… read more here.
Keywords: specific pcr; eight squid; pcr assay; squid species ... See more keywords
Identification of S genotypes in loquat (Eriobotrya japonica Lindl.) based on allele specific PCR
Sign Up to like & getrecommendations! Published in 2017 at "Scientia Horticulturae"
DOI: 10.1016/j.scienta.2017.07.050
Abstract: Abstract Loquat (Eriobotrya japonica Lindl) is an evergreen fruit tree and belongs to Maloideae in the Rosaceae, which carries the RNase-dependent gametophytic self-incompatibility system (GSI). Use of appropriate pollinator trees is essential for increasing fruit… read more here.
Keywords: specific pcr; loquat eriobotrya; eriobotrya japonica; allele specific ... See more keywords
Development and application of an allele-specific PCR assay for detecting T409C mutation of cyp51 gene linked with tebuconazole resistance in Villosiclava virens (rice false smut)
Sign Up to like & getrecommendations! Published in 2017 at "Canadian Journal of Plant Pathology"
DOI: 10.1080/07060661.2017.1356873
Abstract: Abstract Villosiclava virens is an ascomycete fungus (anamorph: Ustilaginoidea virens) recognized as one of the most economically important pathogens on rice in China that causes false smut disease. In our study, the V. virens cyp51… read more here.
Keywords: specific pcr; t409c mutation; pcr assay; villosiclava virens ... See more keywords
Pathovar-Specific PCR Method for Detection and Identification of Xanthomonas translucens pv. undulosa.
Sign Up to like & getrecommendations! Published in 2023 at "Plant disease"
DOI: 10.1094/pdis-11-22-2677-sr
Abstract: Bacterial leaf streak disease caused by Xanthomonas translucens pv. undulosa is an economically important disease threatening wheat and barley crops around the globe. So far, specific PCR-based detection and identification tests for X. translucens pathovars… read more here.
Keywords: translucens undulosa; xanthomonas translucens; detection identification; detection ... See more keywords
Design of SARS-CoV-2 Variant-Specific PCR Assays Considering Regional and Temporal Characteristics
Sign Up to like & getrecommendations! Published in 2022 at "Applied and Environmental Microbiology"
DOI: 10.1128/aem.02289-21
Abstract: Monitoring the introduction and prevalence of variants of concern (VOCs) and variants of interest (VOIs) in a community can help the local authorities make informed public health decisions. PCR assays can be designed to keep… read more here.
Keywords: variant specific; pcr; pcr assays; sars cov ... See more keywords
Detection of Antimalarial Resistance-Associated Mutations in Plasmodium falciparum via a Platform of Allele-Specific PCR Combined with a Gold Nanoparticle-Based Lateral Flow Assay
Sign Up to like & getrecommendations! Published in 2022 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.02535-22
Abstract: Rapid detection of single nucleotide polymorphisms (SNPs) is essential for malaria treatment. Based on the techniques of allele-specific PCR (AS-PCR) and lateral flow assay (LFA), an accurate and powerful platform for SNP detection of pfmdr1… read more here.
Keywords: allele specific; lateral flow; detection; resistance ... See more keywords
A Rapid and Specific Genotyping Platform for Plasmodium falciparum Chloroquine Resistance via Allele-Specific PCR with a Lateral Flow Assay
Sign Up to like & getrecommendations! Published in 2022 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.02719-21
Abstract: Accurate recognition of the mutation and genotype of genes are essential for the treatment of infectious diseases and genetic diseases. Based on the techniques of allele-specific PCR (AS-PCR) and a lateral flow assay (LFA), a… read more here.
Keywords: flow assay; lateral flow; platform; allele specific ... See more keywords
Nanopore sequencing-derived methylation biomarker prediction for methylation-specific PCR in patients with head and neck squamous cell carcinoma
Sign Up to like & getrecommendations! Published in 2025 at "Clinical Epigenetics"
DOI: 10.1186/s13148-025-01960-7
Abstract: DNA methylation of CpG islands is altered in cancer cells. Hypermethylation of single CpG islands in the promoter regions of tumor-suppressor genes occurs already in the early stages of cancer. These methylation changes are cancer-type… read more here.
Keywords: methylation; neck squamous; head neck; methylation specific ... See more keywords
Specific PCR primer designed from genome data for rapid detection of Fusarium oxysporum f. sp. cubense tropical race 4 in the Cavendish banana
Sign Up to like & getrecommendations! Published in 2024 at "PLOS ONE"
DOI: 10.1371/journal.pone.0313358
Abstract: Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc) tropical race 4 (TR4) severely affects banana production worldwide. Thus, specific PCR primers have been developed to rapidly diagnose and monitor Foc TR4-related fusarium wilt… read more here.
Keywords: oxysporum cubense; fusarium; foc tr4; tr4 ... See more keywords