Evaluation of spectral libraries and sample preparation for DIA-LC-MS analysis of host cell proteins: A case study of a bacterially expressed recombinant biopharmaceutical protein.
Sign Up to like & getrecommendations! Published in 2018 at "Protein expression and purification"
DOI: 10.1016/j.pep.2018.03.002
Abstract: Recombinantly expressed biopharmaceutical proteins often undergo a series of purification steps with the aim of removing contaminating material. Depending on the application of the protein, there are various requirements for the degree of purity, but… read more here.
Keywords: protein; biopharmaceutical protein; host cell; spectral libraries ... See more keywords
MSLibrarian: Optimized Predicted Spectral Libraries for Data-Independent Acquisition Proteomics
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Proteome Research"
DOI: 10.1021/acs.jproteome.1c00796
Abstract: Data-independent acquisition-mass spectrometry (DIA-MS) is the method of choice for deep, consistent, and accurate single-shot profiling in bottom-up proteomics. While classic workflows for targeted quantification from DIA-MS data require auxiliary data-dependent acquisition (DDA) MS analysis… read more here.
Keywords: spectral libraries; data independent; optimized predicted; dia ... See more keywords
Building Spectral Libraries from Narrow-Window Data-Independent Acquisition Mass Spectrometry Data.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.1c00895
Abstract: Advances in library-based methods for peptide detection from data-independent acquisition (DIA) mass spectrometry have made it possible to detect and quantify tens of thousands of peptides in a single mass spectrometry run. However, many of… read more here.
Keywords: spectral libraries; data independent; acquisition; mass spectrometry ... See more keywords
DIALib-QC an assessment tool for spectral libraries in data-independent acquisition proteomics
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-18901-y
Abstract: Data-independent acquisition (DIA) mass spectrometry, also known as Sequential Window Acquisition of all Theoretical Mass Spectra (SWATH), is a popular label-free proteomics strategy to comprehensively quantify peptides/proteins utilizing mass spectral libraries to decipher inherently multiplexed… read more here.
Keywords: data independent; mass; tool; independent acquisition ... See more keywords
Memory-Efficient Searching of Gas-Chromatography Mass Spectra Accelerated by Prescreening
Sign Up to like & getrecommendations! Published in 2022 at "Metabolites"
DOI: 10.3390/metabo12060491
Abstract: The number of metabolomics studies and spectral libraries for compound annotation (i.e., assigning possible compound identities to a fragmentation spectrum) has been growing steadily in recent years. Accompanying this growth is the number of mass… read more here.
Keywords: spectral libraries; mass spectra; compound annotation; memory ... See more keywords
Combining Feature-Based Molecular Networking and Contextual Mass Spectral Libraries to Decipher Nutrimetabolomics Profiles
Sign Up to like & getrecommendations! Published in 2022 at "Metabolites"
DOI: 10.3390/metabo12101005
Abstract: Untargeted metabolomics approaches deal with complex data hindering structural information for the comprehensive analysis of unknown metabolite features. We investigated the metabolite discovery capacity and the possible extension of the annotation coverage of the Feature-Based… read more here.
Keywords: spectral libraries; contextual mass; mass spectral; annotation ... See more keywords