Determination of Intracellular Esterase Activity Using Ratiometric Raman Sensing and Spectral Phasor Analysis
Sign Up to like & getrecommendations! Published in 2023 at "Analytical Chemistry"
DOI: 10.1021/acs.analchem.2c05708
Abstract: Carboxylesterases (CEs) are a class of enzymes that catalyze the hydrolysis of esters in a variety of endogenous and exogenous molecules. CEs play an important role in drug metabolism, in the onset and progression of… read more here.
Keywords: spectral phasor; microscopy; raman; activity using ... See more keywords
Label-Free Cytometric Evaluation of Mitosis via Stimulated Raman Scattering Microscopy and Spectral Phasor Analysis
Sign Up to like & getrecommendations! Published in 2023 at "Analytical Chemistry"
DOI: 10.1021/acs.analchem.3c00212
Abstract: Hyperspectral stimulated Raman scattering (SRS) microscopy is a robust imaging tool for the analysis of biological systems. Here, we present a unique perspective, a label-free spatiotemporal map of mitosis, by integrating hyperspectral SRS microscopy with… read more here.
Keywords: label free; mitosis; spectral phasor; microscopy ... See more keywords
Spectral phasor imaging on a commercial confocal microscope without a spectral detector
Sign Up to like & getrecommendations! Published in 2025 at "Scientific Reports"
DOI: 10.1038/s41598-025-15637-x
Abstract: Spectral imaging is a fluorescence microscopy technique with several applications, including imaging of environment-sensitive probes, spectral unmixing and identification of fluorescent species. In confocal microscopes not equipped with a spectral detection unit, spectral images can… read more here.
Keywords: spectral detector; microscope; spectral phasor; commercial confocal ... See more keywords
Stimulated Raman scattering microscopy with spectral phasor analysis: applications in assessing drug–cell interactions
Sign Up to like & getrecommendations! Published in 2022 at "Chemical Science"
DOI: 10.1039/d1sc06976d
Abstract: Statins have displayed significant, although heterogeneous, anti-tumour activity in breast cancer disease progression and recurrence. They offer promise as a class of drugs, normally used for cardiovascular disease control, that could have a significant impact… read more here.
Keywords: cell; spectral phasor; microscopy; drug ... See more keywords