High-speed atomic force microscopy and its future prospects
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Reviews"
DOI: 10.1007/s12551-017-0356-5
Abstract: Various techniques have been developed and used to investigate how proteins produce complex biological architectures and phenomena. Among these techniques, high-speed atomic force microscopy (HS-AFM) holds a unique position. It is only HS-AFM that allows… read more here.
Keywords: speed atomic; microscopy; force microscopy; atomic force ... See more keywords
High-speed atomic force microscopy.
Sign Up to like & getrecommendations! Published in 2019 at "Current opinion in chemical biology"
DOI: 10.1016/j.cbpa.2019.05.010
Abstract: High-speed atomic force microscopy (HS-AFM) is a unique tool for molecular imaging. It can directly visualize protein molecules during their functional activity at high spatiotemporal resolution, without a marker being attached to the molecules. Molecular… read more here.
Keywords: speed atomic; microscopy; force microscopy; atomic force ... See more keywords
Dynamics of Inter-Molecular Interactions Between Single Aβ42 Oligomeric and Aggregate Species by High-Speed Atomic Force Microscopy.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of molecular biology"
DOI: 10.1016/j.jmb.2019.04.044
Abstract: Within the amyloid hypothesis in Alzheimer's disease, current focus has shifted to earlier stages of amyloid beta (Aβ) peptide assembly, involving soluble oligomers and smaller aggregates, which are more toxic to cells compared to their… read more here.
Keywords: speed atomic; microscopy; force microscopy; atomic force ... See more keywords
Advances in high-speed atomic force microscopy (HS-AFM) reveal dynamics of transmembrane channels and transporters.
Sign Up to like & getrecommendations! Published in 2019 at "Current opinion in structural biology"
DOI: 10.1016/j.sbi.2019.02.008
Abstract: Recent advances in high-speed atomic force microscopy (HS-AFM) have made it possible to study the conformational dynamics of single unlabeled transmembrane channels and transporters. Improving environmental control with the integration of a non-disturbing buffer exchange… read more here.
Keywords: speed atomic; microscopy; force microscopy; atomic force ... See more keywords
Acquisition and processing of high-speed atomic force microscopy videos for single amyloid aggregate observation.
Sign Up to like & getrecommendations! Published in 2021 at "Methods"
DOI: 10.1016/j.ymeth.2021.06.006
Abstract: The structural dynamics of the amyloid protein aggregation process are associated with neurodegenerative diseases, including Alzheimer's disease and Parkinson's disease. High-speed atomic force microscopy (HS-AFM) is able to visualize the structural dynamics of individual aggregate… read more here.
Keywords: speed atomic; microscopy; force microscopy; atomic force ... See more keywords
The Role of Speed in Atomic Scale Wear
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Physical Chemistry C"
DOI: 10.1021/acs.jpcc.0c09191
Abstract: Decoupling the role of various stress components during atomic scale wear—such as normal, shear, and hoop stresses—remains one of the biggest challenges preventing a full understanding of how wear ... read more here.
Keywords: role speed; atomic scale; speed atomic; chemistry ... See more keywords
High-Speed Atomic Force Microscopy Reveals Spontaneous Nucleosome Sliding of H2A.Z at the Subsecond Time Scale.
Sign Up to like & getrecommendations! Published in 2023 at "Nano letters"
DOI: 10.1021/acs.nanolett.2c04346
Abstract: Nucleosome dynamics, such as nucleosome sliding and DNA unwrapping, are important for gene regulation in eukaryotic chromatin. H2A.Z, a variant of histone H2A that is highly evolutionarily conserved, participates in gene regulation by forming unstable… read more here.
Keywords: high speed; speed atomic; microscopy; nucleosome sliding ... See more keywords
Structural Dynamics of Amyloid-β Protofibrils and Actions of Anti-Amyloid-β Antibodies as Observed by High-Speed Atomic Force Microscopy.
Sign Up to like & getrecommendations! Published in 2023 at "Nano letters"
DOI: 10.1021/acs.nanolett.3c00187
Abstract: Amyloid-β (Aβ) aggregation intermediates, including oligomers and protofibrils (PFs), have attracted attention as neurotoxic aggregates in Alzheimer's disease. However, due to the complexity of the aggregation pathway, the structural dynamics of aggregation intermediates and how… read more here.
Keywords: high speed; speed atomic; force microscopy; microscopy ... See more keywords
High-Speed Atomic Force Microscopy Visualization of the Dynamics of the Multienzyme Fatty Acid Synthase.
Sign Up to like & getrecommendations! Published in 2017 at "ACS nano"
DOI: 10.1021/acsnano.7b04216
Abstract: Multienzymes, such as the protein metazoan fatty acid synthase (FAS), are giant and highly dynamic molecular machines for critical biosynthetic processes. The molecular architecture of FAS was elucidated by static high-resolution crystallographic analysis, while electron… read more here.
Keywords: speed atomic; microscopy; fatty acid; acid synthase ... See more keywords
Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopy
Sign Up to like & getrecommendations! Published in 2017 at "Nature Communications"
DOI: 10.1038/s41467-017-01466-8
Abstract: The CRISPR-associated endonuclease Cas9 binds to a guide RNA and cleaves double-stranded DNA with a sequence complementary to the RNA guide. The Cas9–RNA system has been harnessed for numerous applications, such as genome editing. Here… read more here.
Keywords: real time; speed atomic; microscopy; crispr cas9 ... See more keywords
Multiple dimeric structures and strand-swap dimerization of E-cadherin in solution visualized by high-speed atomic force microscopy
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2208067119
Abstract: Significance E-cadherin is a major cell–cell adhesion molecule that connects opposing cells via extracellular domains (ectodomains) and regulates tissue morphology and dynamics. Mutual binding of the ectodomains has been considered to be formed by a… read more here.
Keywords: high speed; speed atomic; microscopy; cadherin ... See more keywords