KIF2C affects sperm cell differentiation in patients with Klinefelter syndrome, as revealed by RNA‐Seq and scRNA‐Seq data
Sign Up to like & getrecommendations! Published in 2022 at "FEBS Open Bio"
DOI: 10.1002/2211-5463.13446
Abstract: Klinefelter syndrome (KS) is a leading contributor to male infertility and is characterised by complex and diverse clinical features; however, genetic changes in the KS transcriptome remain largely unknown. We therefore used transcriptomic and single‐cell… read more here.
Keywords: cell; scrna seq; seq; klinefelter syndrome ... See more keywords
Multifunctional 4D‐Printed Sperm‐Hybrid Microcarriers for Assisted Reproduction (Adv. Mater. 50/2022)
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Materials"
DOI: 10.1002/adma.202204257
Abstract: Remotely controllable microrobots are appealing for various biomedical in vivo applications. In particular, our group has focused in the last years in developing sperm-microcarriers to assist sperm cells with motion deficiencies or low count (two… read more here.
Keywords: multifunctional printed; sperm hybrid; sperm cells; printed sperm ... See more keywords
Stain-free interferometric phase microscopy correlation with DNA fragmentation stain in human spermatozoa.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of biophotonics"
DOI: 10.1002/jbio.201800137
Abstract: Acridine orange (AO) staining is used to diagnose DNA fragmentation status in sperm cells. Interferometric phase microscopy (IPM) is an optical imaging method based on digital holographic microscopy that provides quantitative morphological and refractive index… read more here.
Keywords: microscopy; sperm cells; dna fragmentation; fragmentation ... See more keywords
Using Herbal dyes as an alternative staining method for sperm evaluation
Sign Up to like & getrecommendations! Published in 2020 at "Veterinary Medicine and Science"
DOI: 10.1002/vms3.268
Abstract: Abstract Staining, as a valuable method for sperm morphological assessment, has been used to determine sperm abnormalities, fertilization capability and sperm suitability during freezing‐thawing process. Synthetic dyes have been used for sperm viability and morphological… read more here.
Keywords: viability; sperm cells; evaluation; sperm staining ... See more keywords
Isolation of the male germ unit: organization and function in tobacco (Nicotiana tabacum L.)
Sign Up to like & getrecommendations! Published in 2019 at "Plant Cell Reports"
DOI: 10.1007/s002990050547
Abstract: Abstract Sperm cells are released from pollen tubes of tobacco as linked cells, associated with the vegetative nucleus in an assemblage known as the male germ unit (MGU). Using light microscopy, the MGU assemblage appears… read more here.
Keywords: tobacco; microscopy; sperm cells; germ unit ... See more keywords
Selection and Characterization of DNA Aptamers Targeting hLCN6 Protein for Sperm Capture
Sign Up to like & getrecommendations! Published in 2022 at "Applied Biochemistry and Biotechnology"
DOI: 10.1007/s12010-022-03834-7
Abstract: It is an urgent and difficult task to establish a simple and efficient method for identifying and isolating sperm cells from mixed stains in forensic science. In this project, we developed a DNA aptamer-based system… read more here.
Keywords: dna; sperm cells; protein; capture ... See more keywords
Reproductive performance in gilts following applications of different insemination doses and techniques.
Sign Up to like & getrecommendations! Published in 2020 at "Theriogenology"
DOI: 10.1016/j.theriogenology.2020.10.030
Abstract: This study evaluated the applicability of intrauterine artificial insemination (IUAI) in gilts and the impact of age at insemination and different body characteristics of gilts on the success rate for cannula insertion. Additionally, reproductive performance… read more here.
Keywords: reproductive performance; cannula insertion; sperm cells; rate ... See more keywords
Separation of sperm cells from samples containing high concentrations of white blood cells using a spiral channel.
Sign Up to like & getrecommendations! Published in 2017 at "Biomicrofluidics"
DOI: 10.1063/1.4994548
Abstract: Microfluidic technology has potential to separate sperm cells from unwanted debris while improving the effectiveness of assisted reproductive technologies (ART). Current clinical protocol limitations regarding the separation of sperm cells from other cells/cellular debris can… read more here.
Keywords: spiral channel; white blood; sperm cells; sperm ... See more keywords
Successful extraction of sperm cells after autologous bone marrow transplant: a case report
Sign Up to like & getrecommendations! Published in 2019 at "Scandinavian Journal of Urology"
DOI: 10.1080/21681805.2019.1570329
Abstract: Abstract We present a case where microdissection testicular sperm extraction (m.T.E.S.E.) was used in a 36-year-old man after non-Hodgkin T-cell lymphoma treated with 2.5 years of chemotherapy, radiation therapy and autologous bone marrow transplantation (B.M.T.).… read more here.
Keywords: extraction; autologous bone; case; sperm cells ... See more keywords
Human sperm cells can form paracetamol metabolite AM404 that directly interferes with sperm calcium signalling and function through a CatSper-dependent mechanism.
Sign Up to like & getrecommendations! Published in 2022 at "Human reproduction"
DOI: 10.1093/humrep/deac042
Abstract: STUDY QUESTION Do paracetamol (N-acetyl-para-aminophenol (APAP) or acetaminophen) and/or its metabolites affect human sperm Ca2+-signalling and function? SUMMARY ANSWER While APAP itself does not interact with Ca2+-signalling in human sperm, its metabolite N-arachidonoyl phenolamine (AM404),… read more here.
Keywords: function; apap; am404; sperm cells ... See more keywords
Automatic Tracking and Motility Analysis of Human Sperm in Time-Lapse Images
Sign Up to like & getrecommendations! Published in 2017 at "IEEE Transactions on Medical Imaging"
DOI: 10.1109/tmi.2016.2630720
Abstract: We present a fully automated multi-sperm tracking algorithm. It has the demonstrated capability to detect and track simultaneously hundreds of sperm cells in recorded videos while accurately measuring motility parameters over time and with minimal… read more here.
Keywords: human sperm; automatic tracking; time; sperm cells ... See more keywords