Coacervation-Mediated Cytocompatible Formation of Supramolecular Hydrogels with Self-evolving Macropores for 3D Multicellular Spheroid Culture.
Sign Up to like & getrecommendations! Published in 2023 at "Advanced materials"
DOI: 10.1002/adma.202300636
Abstract: Coacervation driven liquid-liquid phase separation of biopolymers has aroused considerable attention for diverse applications, especially for the construction of microstructured polymeric materials. Herein, a coacervate-to-hydrogel transition strategy is developed to create macroporous hydrogels (MPH), which… read more here.
Keywords: self evolving; multicellular spheroid; coacervation; formation ... See more keywords
Spheroid Culture System Confers Differentiated Transcriptome Profile and Functional Advantage to 3T3-L1 Adipocytes
Sign Up to like & getrecommendations! Published in 2018 at "Annals of Biomedical Engineering"
DOI: 10.1007/s10439-018-1993-y
Abstract: This study highlights functional differences between 2-D monolayer and 3-D spheroid 3T3-L1 adipocyte culture models and explores the underlying genomic mechanisms responsible for the different phenotypes present. The spheroids showed higher triglyceride accumulation than the… read more here.
Keywords: confers differentiated; spheroid culture; system confers; culture ... See more keywords
Three-Dimensional Spheroid Culture of Human Mesenchymal Stem Cells: Offering Therapeutic Advantages and In Vitro Glimpses of the In Vivo State
Sign Up to like & getrecommendations! Published in 2023 at "Stem Cells Translational Medicine"
DOI: 10.1093/stcltm/szad011
Abstract: Abstract As invaluable as the standard 2-dimensional (2D) monolayer in vitro cell culture system has been, there is increasing evidence that 3-dimensional (3D) non-adherent conditions are more relevant to the in vivo condition. While one… read more here.
Keywords: culture; cell; human mesenchymal; stem cells ... See more keywords
A three-dimensional spheroid co-culture system of neurons and astrocytes derived from Alzheimer's disease patients for drug efficacy testing.
Sign Up to like & getrecommendations! Published in 2023 at "Cell proliferation"
DOI: 10.1111/cpr.13399
Abstract: Cell culture systems derived from the progenitor cells of human patients have many advantages over animal models for therapeutic drug testing and studies of disease pathogenesis. Here we describe a three-dimensional (3D) spheroid co-culture system… read more here.
Keywords: culture; neurons astrocytes; dimensional spheroid; three dimensional ... See more keywords
An Improved Scalable Hydrogel Dish for Spheroid Culture
Sign Up to like & getrecommendations! Published in 2021 at "Life"
DOI: 10.3390/life11060517
Abstract: Research in fields studying cellular response to surface tension and mechanical forces necessitate cell culture tools with tunability of substrate stiffness. We created a scalable hydrogel dish design to facilitate scaffold-free formation of multiple spheroids… read more here.
Keywords: improved scalable; spheroid culture; dish spheroid; scalable hydrogel ... See more keywords