An Attachment-Independent Biochemical Timer of the Spindle Assembly Checkpoint.
Sign Up to like & getrecommendations! Published in 2017 at "Molecular cell"
DOI: 10.1016/j.molcel.2017.10.011
Abstract: The spindle assembly checkpoint (SAC) generates a diffusible protein complex that prevents anaphase until all chromosomes are properly attached to spindle microtubules. A key step in SAC initiation is the recruitment of MAD1 to kinetochores,… read more here.
Keywords: attachment independent; spindle assembly; timer; biochemical timer ... See more keywords
The Spindle Assembly Checkpoint Is Required for Hematopoietic Progenitor Cell Engraftment
Sign Up to like & getrecommendations! Published in 2017 at "Stem Cell Reports"
DOI: 10.1016/j.stemcr.2017.09.017
Abstract: Summary The spindle assembly checkpoint plays a pivotal role in preventing aneuploidy and transformation. Many studies demonstrate impairment of this checkpoint in cancer cells. While leukemia is frequently driven by transformed hematopoietic stem and progenitor… read more here.
Keywords: spindle assembly; engraftment; checkpoint; progenitor ... See more keywords
Mitotic spindle assembly in animal cells: a fine balancing act
Sign Up to like & getrecommendations! Published in 2017 at "Nature Reviews Molecular Cell Biology"
DOI: 10.1038/nrm.2016.162
Abstract: The mitotic spindle has a crucial role in ensuring the accurate segregation of chromosomes into the two daughter cells during cell division, which is paramount for maintaining genome integrity. It is a self-organized and dynamic… read more here.
Keywords: animal cells; spindle assembly; assembly animal; cells fine ... See more keywords
The long noncoding RNA CRYBG3 induces aneuploidy by interfering with spindle assembly checkpoint via direct binding with Bub3
Sign Up to like & getrecommendations! Published in 2021 at "Oncogene"
DOI: 10.1038/s41388-020-01601-8
Abstract: Aneuploidy is a hallmark of genomic instability that leads to tumor initiation, progression, and metastasis. CDC20, Bub1, and Bub3 form the mitosis checkpoint complex (MCC) that binds the anaphase-promoting complex or cyclosome (APC/C), a crucial… read more here.
Keywords: spindle assembly; crybg3; lnc crybg3; bub3 ... See more keywords
Cdk1-mediated DIAPH1 phosphorylation maintains metaphase cortical tension and inactivates the spindle assembly checkpoint at anaphase
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-019-08957-w
Abstract: Animal cells undergo rapid rounding during mitosis, ensuring proper chromosome segregation, during which an outward rounding force abruptly increases upon prometaphase entry and is maintained at a constant level during metaphase. Initial cortical tension is… read more here.
Keywords: cortical tension; spindle assembly; cdk1 mediated; tension ... See more keywords
Bub1 kinase in the regulation of mitosis
Sign Up to like & getrecommendations! Published in 2021 at "Animal Cells and Systems"
DOI: 10.1080/19768354.2021.1884599
Abstract: ABSTRACT Accurate chromosome segregation is required for cell survival and organismal development. During mitosis, the spindle assembly checkpoint acts as a safeguard to maintain the high fidelity of mitotic chromosome segregation by monitoring the attachment… read more here.
Keywords: bub1; mitosis; spindle assembly; bub1 kinase ... See more keywords
Inositol 1, 4, 5-trisphosphate receptor is required for spindle assembly in Xenopus oocytes
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Biology of the Cell"
DOI: 10.1091/mbc.e22-06-0218
Abstract: The extent to which calcium signaling participates in specific events of animal cell meiosis or mitosis is a subject of enduring controversy. We have previously demonstrated that buffering intracellular calcium with 1,2-bis(2-aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid (BAPTA, a… read more here.
Keywords: calcium; inositol trisphosphate; spindle assembly; xenopus oocytes ... See more keywords
ZmPRD1 is essential for DSB formation, but not required for bipolar spindle assembly during maize meiosis.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of experimental botany"
DOI: 10.1093/jxb/erac075
Abstract: Homologs of PUTATIVE RECOMBINATION INITIATION DEFECT 1 (PRD1) are essential for meiotic double-strand break (DSB) formation in mouse (Mus musculus), Arabidopsis (Arabidopsis thaliana), and rice (Oryza sativa). Latest work reveals that the rice PRD1 also… read more here.
Keywords: bipolar spindle; spindle assembly; meiosis; dsb formation ... See more keywords
Vitrification-induced activation of lysosomal cathepsin B perturbs spindle assembly checkpoint function in mouse oocytes.
Sign Up to like & getrecommendations! Published in 2020 at "Molecular human reproduction"
DOI: 10.1093/molehr/gaaa051
Abstract: As the age of child-bearing increases and correlates with infertility, cryopreservation of female gametes is becoming common-place in ART. However, the developmental competence of vitrified oocytes has remained low. The underlying mechanisms responsible for reduced… read more here.
Keywords: spindle assembly; function; vitrified oocytes; assembly checkpoint ... See more keywords
Physical determinants of bipolar mitotic spindle assembly and stability in fission yeast
Sign Up to like & getrecommendations! Published in 2017 at "Science Advances"
DOI: 10.1126/sciadv.1601603
Abstract: A physical model that exhibits de novo bipolar spindle assembly is used to study the mechanisms of spindle bipolarity. Mitotic spindles use an elegant bipolar architecture to segregate duplicated chromosomes with high fidelity. Bipolar spindles… read more here.
Keywords: spindle; stability; fission yeast; spindle assembly ... See more keywords
The HIPK2/CDC14B-MeCP2 axis enhances the spindle assembly checkpoint block by promoting cyclin B translation
Sign Up to like & getrecommendations! Published in 2023 at "Science Advances"
DOI: 10.1126/sciadv.add6982
Abstract: Mitotic perturbations activate the spindle assembly checkpoint (SAC) that keeps cells in prometaphase with high CDK1 activity. Prolonged mitotic arrest is eventually bypassed by gradual cyclin B decline followed by slippage of cells into G1… read more here.
Keywords: mecp2; spindle assembly; assembly checkpoint; hipk2 cdc14b ... See more keywords