An Attachment-Independent Biochemical Timer of the Spindle Assembly Checkpoint.
Sign Up to like & getrecommendations! Published in 2017 at "Molecular cell"
DOI: 10.1016/j.molcel.2017.10.011
Abstract: The spindle assembly checkpoint (SAC) generates a diffusible protein complex that prevents anaphase until all chromosomes are properly attached to spindle microtubules. A key step in SAC initiation is the recruitment of MAD1 to kinetochores,… read more here.
Keywords: attachment independent; spindle assembly; timer; biochemical timer ... See more keywords
The Spindle Assembly Checkpoint Is Required for Hematopoietic Progenitor Cell Engraftment
Sign Up to like & getrecommendations! Published in 2017 at "Stem Cell Reports"
DOI: 10.1016/j.stemcr.2017.09.017
Abstract: Summary The spindle assembly checkpoint plays a pivotal role in preventing aneuploidy and transformation. Many studies demonstrate impairment of this checkpoint in cancer cells. While leukemia is frequently driven by transformed hematopoietic stem and progenitor… read more here.
Keywords: spindle assembly; engraftment; checkpoint; progenitor ... See more keywords
Mitotic spindle assembly in animal cells: a fine balancing act
Sign Up to like & getrecommendations! Published in 2017 at "Nature Reviews Molecular Cell Biology"
DOI: 10.1038/nrm.2016.162
Abstract: The mitotic spindle has a crucial role in ensuring the accurate segregation of chromosomes into the two daughter cells during cell division, which is paramount for maintaining genome integrity. It is a self-organized and dynamic… read more here.
Keywords: animal cells; spindle assembly; assembly animal; cells fine ... See more keywords
The long noncoding RNA CRYBG3 induces aneuploidy by interfering with spindle assembly checkpoint via direct binding with Bub3
Sign Up to like & getrecommendations! Published in 2021 at "Oncogene"
DOI: 10.1038/s41388-020-01601-8
Abstract: Aneuploidy is a hallmark of genomic instability that leads to tumor initiation, progression, and metastasis. CDC20, Bub1, and Bub3 form the mitosis checkpoint complex (MCC) that binds the anaphase-promoting complex or cyclosome (APC/C), a crucial… read more here.
Keywords: spindle assembly; crybg3; lnc crybg3; bub3 ... See more keywords
Cdk1-mediated DIAPH1 phosphorylation maintains metaphase cortical tension and inactivates the spindle assembly checkpoint at anaphase
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-019-08957-w
Abstract: Animal cells undergo rapid rounding during mitosis, ensuring proper chromosome segregation, during which an outward rounding force abruptly increases upon prometaphase entry and is maintained at a constant level during metaphase. Initial cortical tension is… read more here.
Keywords: cortical tension; spindle assembly; cdk1 mediated; tension ... See more keywords
C. elegans SSNA-1 is required for the structural integrity of centrioles and bipolar spindle assembly
Sign Up to like & getrecommendations! Published in 2025 at "Nature Communications"
DOI: 10.1038/s41467-025-59939-0
Abstract: Centrioles play key roles in mitotic spindle assembly. Once assembled, centrioles exhibit long-term stability, but how stability is achieved and how it is regulated are not completely understood. In this study we show that SSNA-1,… read more here.
Keywords: ssna; structural integrity; stability; spindle assembly ... See more keywords
A coadapted KNL1 and spindle assembly checkpoint axis orchestrates precise mitosis in Arabidopsis
Sign Up to like & getrecommendations! Published in 2024 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2316583121
Abstract: Significance As a scaffold for kinetochore assembly, kinetochore scaffold 1 (KNL1) functions in recruiting spindle assembly checkpoint (SAC) components among many well-studied eukaryotes from yeasts to humans. Plants have acquired many unique features associated with… read more here.
Keywords: assembly checkpoint; mitosis; spindle assembly; checkpoint ... See more keywords
The Arabidopsis BUB1/MAD3 family protein BMF3 requires BUB3.3 to recruit CDC20 to kinetochores in spindle assembly checkpoint signaling
Sign Up to like & getrecommendations! Published in 2024 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2322677121
Abstract: Significance Mitotic progression into anaphase is monitored by the spindle assembly checkpoint that is poorly understood in plants. Using Arabidopsis thaliana as a model system, we identified an interaction pattern centered at the BUB1 and… read more here.
Keywords: family protein; bub1 mad3; protein bmf3; assembly checkpoint ... See more keywords
Bub1 kinase in the regulation of mitosis
Sign Up to like & getrecommendations! Published in 2021 at "Animal Cells and Systems"
DOI: 10.1080/19768354.2021.1884599
Abstract: ABSTRACT Accurate chromosome segregation is required for cell survival and organismal development. During mitosis, the spindle assembly checkpoint acts as a safeguard to maintain the high fidelity of mitotic chromosome segregation by monitoring the attachment… read more here.
Keywords: bub1; mitosis; spindle assembly; bub1 kinase ... See more keywords
Inositol 1, 4, 5-trisphosphate receptor is required for spindle assembly in Xenopus oocytes
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Biology of the Cell"
DOI: 10.1091/mbc.e22-06-0218
Abstract: The extent to which calcium signaling participates in specific events of animal cell meiosis or mitosis is a subject of enduring controversy. We have previously demonstrated that buffering intracellular calcium with 1,2-bis(2-aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid (BAPTA, a… read more here.
Keywords: calcium; inositol trisphosphate; spindle assembly; xenopus oocytes ... See more keywords
O-067 Biallelic variants in DLGAP5 cause spindle assembly defects and human early embryonic arrest
Sign Up to like & getrecommendations! Published in 2025 at "Human Reproduction"
DOI: 10.1093/humrep/deaf097.067
Abstract: What effects do DLGAP5 defects have on human early embryo development? DLGAP5 deficiency disrupts normal spindle assembly through its interaction with TACC3, resulting in female infertility characterized as recurrent early embryonic arrest (REEA). REEA is… read more here.
Keywords: human early; defects human; spindle assembly; early embryonic ... See more keywords