A Cell-Based Assay for Mitotic Spindle Orientation.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7847-2_5
Abstract: The regulation of mitotic spindle orientation is essential to ensure proper cell division and development (Kiyomitsua and Cheeseman Nat Cell Biol 14:311-317, 2012). For identification of potential spindle orientation regulators, determination of the mitotic spindle… read more here.
Keywords: spindle orientation; cell based; mitotic spindle; based assay ... See more keywords
Morphogenetic forces planar polarize LGN/Pins in the embryonic head during Drosophila gastrulation
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DOI: 10.1101/2022.01.07.475359
Abstract: Spindle orientation is often achieved by a complex of Pins/LGN, Mud/NuMa, Gαi, and Dynein, which interacts with astral microtubules to rotate the spindle. Cortical Pins/LGN recruitment serves as a critical step in this process. Here,… read more here.
Keywords: spindle orientation; drosophila; orientation; gastrulation ... See more keywords
PP2A-B55γ counteracts Cdk1 and regulates proper spindle orientation through the cortical dynein adaptor NuMA
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DOI: 10.1242/jcs.243857
Abstract: ABSTRACT Proper orientation of the mitotic spindle is critical for accurate development and morphogenesis. In human cells, spindle orientation is regulated by the evolutionarily conserved protein NuMA, which interacts with dynein and enriches it at… read more here.
Keywords: spindle orientation; b55; pp2a b55; t2055 ... See more keywords
Annexin A2 and Ahnak control cortical NuMA-dynein localization and mitotic spindle orientation.
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DOI: 10.1242/jcs.259344
Abstract: Proper mitotic spindle orientation depends on the correct anchorage of astral microtubules to the cortex. It relies on the remodeling of the cell cortex, a process not fully understood. Annexin A2 (Anx2) is a protein… read more here.
Keywords: cell; localization; ahnak; spindle orientation ... See more keywords
Regulated spindle orientation buffers tissue growth in the epidermis
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DOI: 10.7554/elife.48482
Abstract: Tissue homeostasis requires a balance between progenitor cell proliferation and loss. Mechanisms that maintain this robust balance are needed to avoid tissue loss or overgrowth. Here we demonstrate that regulation of spindle orientation/asymmetric cell divisions… read more here.
Keywords: orientation; cell divisions; asymmetric cell; spindle orientation ... See more keywords