Roles of minor spliceosome in intron recognition and the convergence with the better understood major spliceosome
Sign Up to like & getrecommendations! Published in 2022 at "Wiley Interdisciplinary Reviews: RNA"
DOI: 10.1002/wrna.1761
Abstract: Catalyzed by spliceosomes in the nucleus, RNA splicing removes intronic sequences from precursor RNAs in eukaryotes to generate mature RNA, which also significantly increases proteome complexity and fine‐tunes gene expression. Most metazoans have two coexisting… read more here.
Keywords: recognition; intron; minor spliceosome; spliceosome ... See more keywords
Evolution of the U2 Spliceosome for Processing Numerous and Highly Diverse Non-canonical Introns in the Chordate Fritillaria borealis
Sign Up to like & getrecommendations! Published in 2019 at "Current Biology"
DOI: 10.1016/j.cub.2019.07.092
Abstract: An overwhelming majority of eukaryotic introns have GT/AG ends, whose identities play a critical role for their recognition and removal by the U2 spliceosome, a well-conserved complex of protein and RNAs. Introns with other splice… read more here.
Keywords: chordate fritillaria; non canonical; spliceosome; canonical introns ... See more keywords
Genetics and biochemistry remain essential in the structural era of the spliceosome.
Sign Up to like & getrecommendations! Published in 2017 at "Methods"
DOI: 10.1016/j.ymeth.2017.01.006
Abstract: The spliceosome is not a single macromolecular machine. Rather it is a collection of dynamic heterogeneous subcomplexes that rapidly interconvert throughout the course of a typical splicing cycle. Because of this, for many years the… read more here.
Keywords: biochemistry; remain essential; biochemistry remain; genetics ... See more keywords
Mutant U2AF1-expressing cells are sensitive to pharmacological modulation of the spliceosome
Sign Up to like & getrecommendations! Published in 2017 at "Nature Communications"
DOI: 10.1038/ncomms14060
Abstract: Somatic mutations in spliceosome genes are detectable in ∼50% of patients with myelodysplastic syndromes (MDS). We hypothesize that cells harbouring spliceosome gene mutations have increased sensitivity to pharmacological perturbation of the spliceosome. We focus on… read more here.
Keywords: u2af1 expressing; mutant u2af1; expressing cells; u2af1 s34f ... See more keywords
CBMT-38. THERAPEUTIC INHIBITION OF THE SPLICEOSOME IN GLIOBLASTOMA: CHANGES FORM MINOR TO MAJOR
Sign Up to like & getrecommendations! Published in 2019 at "Neuro-Oncology"
DOI: 10.1093/neuonc/noz175.160
Abstract: Chemical or genetic perturbation of the spliceosome function may represent a therapeutic opportunity for Glioblastoma multiforme (GBM) and other cancers. We and others have shown that oncogenic MYC activity causes spliceosome vulnerability and leads to… read more here.
Keywords: therapeutic inhibition; glioblastoma; inhibition spliceosome; cbmt therapeutic ... See more keywords
Postcatalytic spliceosome structure reveals mechanism of 3′–splice site selection
Sign Up to like & getrecommendations! Published in 2017 at "Science"
DOI: 10.1126/science.aar3729
Abstract: Understanding splicing from the 3′ end The spliceosome removes introns from eukaryotic mRNA precursors and yields mature transcripts by joining exons. Despite decades of functional studies and recent progress in understanding the spliceosome structure, the… read more here.
Keywords: structure; splice site; spliceosome; microscopy ... See more keywords