Sensitive Detection of Small-Molecule Targets Using Cooperative Binding Split Aptamers and Enzyme-Assisted Target Recycling.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.7b03625
Abstract: Signal amplification via enzyme-assisted target recycling (EATR) offers a powerful means for improving the sensitivity of DNA detection assays, but it has proven challenging to employ EATR with aptamer-based assays for small-molecule detection due to… read more here.
Keywords: detection; small molecule; target; binding ... See more keywords
Single Molecule Fluorescent Colocalization of Split Aptamers for Ultrasensitive Detection of Biomolecules.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.8b01916
Abstract: Single-molecule fluorescence imaging is a promising strategy for biomolecule detection. However, the accuracy of single-molecule method is often compromised by the false-positive events at the ultralow sample levels that are caused by the nonspecific adsorption… read more here.
Keywords: molecule; detection; single molecule; fluorescent colocalization ... See more keywords
Unconventional Split Aptamers Cleaved at Functionally Essential Sites Preserve Biorecognition Capability.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.9b04115
Abstract: Split aptamers (SPAs) are a pair of oligonucleotide fragments generated by cleaving a long parent aptamer. SPAs have many compelling advantages over the parent aptamer such as sandwich target binding, optimized concise structure, and low… read more here.
Keywords: spas; functionally essential; biorecognition capability; split aptamers ... See more keywords
Assembly of split aptamers by dynamic pH-responsive covalent ligation.
Sign Up to like & getrecommendations! Published in 2023 at "Chemical communications"
DOI: 10.1039/d3cc01158e
Abstract: Reversible pH-responsive N-methoxyoxazolidine formation is used to ligate split aptamer fragments. Two twice-split models and one thrice-split model of CBA (cocaine-binding aptamer) were examined. The aptamer assembly was dynamic, proportional to the substrate concentration and… read more here.
Keywords: split aptamers; responsive covalent; ligation; assembly split ... See more keywords
Split aptamer acquisition mechanisms and current application in antibiotics detection: a short review.
Sign Up to like & getrecommendations! Published in 2022 at "Critical reviews in food science and nutrition"
DOI: 10.1080/10408398.2022.2064810
Abstract: Antibiotic contamination is becoming a prominent global issue. Therefore, sensitive, specific and simple technology is desirable the demand for antibiotics detection. Biosensors based on split aptamer has gradually attracted extensive attention for antibiotic detection due… read more here.
Keywords: antibiotics detection; split aptamers; split aptamer; detection ... See more keywords