High-Throughput Isolation of Soluble Protein Domains Using a Bipartite Split-GFP Complementation System.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9624-7_15
Abstract: The identification of soluble, folded domains of proteins is a recurring task in modern molecular biology. We detail a protocol for identifying compact soluble protein domains using a self-assembling two-part split-GFP comprised of a detector… read more here.
Keywords: protein; split gfp; soluble protein; domains using ... See more keywords
Protein stabilization with retained function of monellin using a split GFP system
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-31177-z
Abstract: Sweet proteins are an unexploited resource in the search for non-carbohydrate sweeteners mainly due to their low stability towards heating. Variants of the sweet protein monellin, with increased stability, were derived by an in vivo screening… read more here.
Keywords: protein; split gfp; monellin; gfp ... See more keywords
FAST, a method based on split-GFP for the detection in solution of proteins synthesized in cell-free expression systems
Sign Up to like & getrecommendations! Published in 2024 at "Scientific Reports"
DOI: 10.1038/s41598-024-58588-5
Abstract: Cell-free protein synthesis (CFPS) systems offer a versatile platform for a wide range of applications. However, the traditional methods for detecting proteins synthesized in CFPS, such as radioactive labeling, fluorescent tagging, or electrophoretic separation, may… read more here.
Keywords: detection; method; proteins synthesized; cell free ... See more keywords
Quantitative measurement of cell-surface displayed proteins based on split-GFP assembly
Sign Up to like & getrecommendations! Published in 2024 at "Microbial Cell Factories"
DOI: 10.1186/s12934-024-02386-1
Abstract: Background Microbial cell surface display technology allows immobilizing proteins on the cell surface by fusing them to anchoring motifs, thereby endowing the cells with diverse functionalities. However, the assessment of successful protein display and the… read more here.
Keywords: split; surface; cell; gfp assembly ... See more keywords
A Split-GFP Gateway Cloning System for Topology Analyses of Membrane Proteins in Plants
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DOI: 10.1371/journal.pone.0170118
Abstract: To understand the function of membrane proteins, it is imperative to know their topology. For such studies, a split green fluorescent protein (GFP) method is useful. GFP is barrel-shaped, consisting of 11 β-sheets. When the… read more here.
Keywords: split gfp; system; membrane proteins; topology ... See more keywords
Tripartite split-GFP assay to identify selective intracellular nanobody that suppresses GTPase RHOA subfamily downstream signaling
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2022.980539
Abstract: Strategies based on intracellular expression of artificial binding domains present several advantages over manipulating nucleic acid expression or the use of small molecule inhibitors. Intracellularly-functional nanobodies can be considered as promising macrodrugs to study key… read more here.
Keywords: gtpase rhoa; tripartite split; split gfp; rhoa subfamily ... See more keywords
Split-GFP Reassembly Assay: Strengths and Caveats from a Multiparametric Analysis
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms232113167
Abstract: The split-Green Fluorescent Protein (GFP) reassembly assay is a powerful approach to study protein–protein interactions (PPIs). In this assay, two proteins, respectively, fused to the first seven and the last four β-strands of GFP are… read more here.
Keywords: assay strengths; gfp; gfp reassembly; split gfp ... See more keywords