High-Throughput Isolation of Soluble Protein Domains Using a Bipartite Split-GFP Complementation System.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9624-7_15
Abstract: The identification of soluble, folded domains of proteins is a recurring task in modern molecular biology. We detail a protocol for identifying compact soluble protein domains using a self-assembling two-part split-GFP comprised of a detector… read more here.
Keywords: protein; split gfp; soluble protein; domains using ... See more keywords
Protein stabilization with retained function of monellin using a split GFP system
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-31177-z
Abstract: Sweet proteins are an unexploited resource in the search for non-carbohydrate sweeteners mainly due to their low stability towards heating. Variants of the sweet protein monellin, with increased stability, were derived by an in vivo screening… read more here.
Keywords: protein; split gfp; monellin; gfp ... See more keywords
A Split-GFP Gateway Cloning System for Topology Analyses of Membrane Proteins in Plants
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DOI: 10.1371/journal.pone.0170118
Abstract: To understand the function of membrane proteins, it is imperative to know their topology. For such studies, a split green fluorescent protein (GFP) method is useful. GFP is barrel-shaped, consisting of 11 β-sheets. When the… read more here.
Keywords: split gfp; system; membrane proteins; topology ... See more keywords
Tripartite split-GFP assay to identify selective intracellular nanobody that suppresses GTPase RHOA subfamily downstream signaling
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2022.980539
Abstract: Strategies based on intracellular expression of artificial binding domains present several advantages over manipulating nucleic acid expression or the use of small molecule inhibitors. Intracellularly-functional nanobodies can be considered as promising macrodrugs to study key… read more here.
Keywords: gtpase rhoa; tripartite split; split gfp; rhoa subfamily ... See more keywords
Split-GFP Reassembly Assay: Strengths and Caveats from a Multiparametric Analysis
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms232113167
Abstract: The split-Green Fluorescent Protein (GFP) reassembly assay is a powerful approach to study protein–protein interactions (PPIs). In this assay, two proteins, respectively, fused to the first seven and the last four β-strands of GFP are… read more here.
Keywords: assay strengths; gfp; gfp reassembly; split gfp ... See more keywords