Novel Split Intein-Mediated Enzymatic Channeling Accelerates the Multimeric Bioconversion Pathway of Ginsenoside.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.2c00216
Abstract: Cascade reaction systems, such as protein fusion and synthetic protein scaffold systems, can both spatially control the metabolic flux and boost the productivity of multistep enzymatic reactions. Despite many efforts to generate fusion proteins, this… read more here.
Keywords: system; split; multimeric bioconversion; intein mediated ... See more keywords
A Conserved Histidine Residue Drives Extein Dependence in an Enhanced Atypically Split Intein.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c08985
Abstract: Split intein-mediated protein trans-splicing (PTS) is widely applied in chemical biology and biotechnology to carry out traceless and specific protein ligation. However, the external residues immediately flanking the intein (exteins) can reduce the splicing rate,… read more here.
Keywords: dependence; dependence enhanced; atypically split; extein dependence ... See more keywords
An Atypical Mechanism of Split Intein Molecular Recognition and Folding.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.8b07334
Abstract: Split inteins associate to trigger protein splicing in trans, a post-translational modification in which protein sequences fused to the intein pair are ligated together in a traceless manner. Recently, a family of naturally split inteins… read more here.
Keywords: protein; split inteins; split intein; split ... See more keywords
Split Intein-Mediated Protein Ligation for detecting protein-protein interactions and their inhibition
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-16299-1
Abstract: Here, to overcome many limitations accompanying current available methods to detect protein-protein interactions (PPIs), we develop a live cell method called Split Intein-Mediated Protein Ligation (SIMPL). In this approach, bait and prey proteins are respectively… read more here.
Keywords: protein; intein mediated; protein interactions; protein protein ... See more keywords
Efficient generation of bispecific IgG antibodies by split intein mediated protein trans-splicing system
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-08641-3
Abstract: Many methods have been developed to produce bispecific antibodies (BsAbs) for industrial application. However, huge challenges still remain in synthesizing whole length BsAbs, including their assembly, stability, immunogenicity, and pharmacodynamics. Here we present for first… read more here.
Keywords: bispecific igg; split intein; protein trans; igg antibodies ... See more keywords
Structural and biochemical analysis of a novel atypically split intein reveals a conserved histidine specific to cysteine-less inteins
Sign Up to like & getrecommendations! Published in 2023 at "Chemical Science"
DOI: 10.1039/d3sc01200j
Abstract: Protein trans-splicing mediated by a split intein reconstitutes a protein backbone from two parts. This virtually traceless autoprocessive reaction provides the basis for numerous protein engineering applications. Protein splicing typically proceeds through two thioester or… read more here.
Keywords: atypically split; histidine; cysteine less; split intein ... See more keywords