Harnessing Split‐Inteins as a Tool for the Selective Modification of Surface Receptors in Live Cells
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202200487
Abstract: Biochemical studies of integral membrane proteins are often hampered by low purification yields and technical limitations such as aggregation causing in vitro manipulations to be challenging. The ability of controlling proteins in live cells bypasses these… read more here.
Keywords: surface; live cells; inteins tool; split inteins ... See more keywords
Promiscuous Trans-splicing Activities Revealed by Next Generation Sequencing-based Analysis of 298 Split Inteins
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology and Bioprocess Engineering"
DOI: 10.1007/s12257-019-0394-x
Abstract: Protein trans-splicing is a naturally occurring process in which two protein fragments are ligated by a reaction between two intein domains, called split inteins. Despite their usefulness in research, the reactivity and structure of only… read more here.
Keywords: split inteins; split; splicing activities; next generation ... See more keywords
An Atypical Mechanism of Split Intein Molecular Recognition and Folding.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.8b07334
Abstract: Split inteins associate to trigger protein splicing in trans, a post-translational modification in which protein sequences fused to the intein pair are ligated together in a traceless manner. Recently, a family of naturally split inteins… read more here.
Keywords: protein; split inteins; split intein; split ... See more keywords