Efficient targeted mutagenesis in Epichloë festucae using a split marker system.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of microbiological methods"
DOI: 10.1016/j.mimet.2016.12.017
Abstract: A split-marker system for targeted gene deletion was developed for the model grass endophytic fungus Epichloë festucae. Compared to the conventional system that yields up to 25% homologous recombinants, the method resulted in 33-74% targeted… read more here.
Keywords: marker system; system; festucae using; epichlo festucae ... See more keywords
Expanding the Toolbox for Functional Genomics in Fonsecaea pedrosoi: The Use of Split-Marker and Biolistic Transformation for Inactivation of Tryptophan Synthase (trpB) Gene
Sign Up to like & getrecommendations! Published in 2023 at "Journal of Fungi"
DOI: 10.3390/jof9020224
Abstract: Chromoblastomycosis (CBM) is a disease caused by several dematiaceous fungi from different genera, and Fonsecaea is the most common which has been clinically isolated. Genetic transformation methods have recently been described; however, molecular tools for… read more here.
Keywords: split marker; fonsecaea pedrosoi; marker biolistic; pedrosoi use ... See more keywords