Sequencing Spo11 Oligonucleotides for Mapping Meiotic DNA Double-Strand Breaks in Yeast.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6340-9_3
Abstract: Meiosis is a specialized form of cell division resulting in reproductive cells with a reduced, usually haploid, genome complement. A key step after premeiotic DNA replication is the occurrence of homologous recombination at multiple places… read more here.
Keywords: dna; sequencing spo11; dna double; strand breaks ... See more keywords
ATM and PRDM9 regulate SPO11-bound recombination intermediates during meiosis
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DOI: 10.1038/s41467-020-14654-w
Abstract: Meiotic recombination is initiated by SPO11-induced double-strand breaks (DSBs). In most mammals, the methyltransferase PRDM9 guides SPO11 targeting, and the ATM kinase controls meiotic DSB numbers. Following MRE11 nuclease removal of SPO11, the DSB is… read more here.
Keywords: prdm9 regulate; spo11 bound; recombination; atm prdm9 ... See more keywords
Concerted cutting by Spo11 illuminates meiotic DNA break mechanics.
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DOI: 10.1038/s41586-021-03389-3
Abstract: Genetic recombination arises during meiosis through the repair of DNA double-strand breaks (DSBs) that are created by Spo11, a topoisomerase-like protein1,2. Spo11 DSBs form preferentially in nucleosome-depleted regions termed hotspots3,4, yet how Spo11 engages with… read more here.
Keywords: dna; cutting spo11; concerted cutting; spo11 dsbs ... See more keywords
Spo11 generates gaps through concerted cuts at sites of topological stress.
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DOI: 10.1038/s41586-021-03632-x
Abstract: Meiotic recombination is essential for chromosome segregation at meiosis and fertility. It is initiated by programmed DNA double-strand breaks (DSBs) introduced by Spo11, a eukaryotic homologue of an archaeal topoisomerase (Topo VIA)1. Here we describe… read more here.
Keywords: topological stress; dna; double dsbs; double dsb ... See more keywords
A segregating human allele of SPO11 modeled in mice disrupts timing and amounts of meiotic recombination, causing oligospermia and a decreased ovarian reserve†
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DOI: 10.1093/biolre/ioz089
Abstract: Abstract A major challenge in medical genetics is to characterize variants of unknown significance (VUS). Doing so would help delineate underlying causes of disease and the design of customized treatments. Infertility has presented an especially… read more here.
Keywords: segregating human; mice; human allele; recombination ... See more keywords
Identification, characterization, and rescue of CRISPR/Cas9 generated wheat SPO11‐1 mutants
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DOI: 10.1111/pbi.13961
Abstract: Summary Increasing crop yields through plant breeding is time consuming and laborious, with the generation of novel combinations of alleles being limited by chromosomal linkage blocks and linkage‐drag. Meiotic recombination is essential to create novel… read more here.
Keywords: wheat; crispr cas9; recombination; spo11 ... See more keywords
Differentiated function and localisation of SPO11-1 and PRD3 on the chromosome axis during meiotic DSB formation in Arabidopsis thaliana
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DOI: 10.1371/journal.pgen.1010298
Abstract: During meiosis, DNA double-strand breaks (DSBs) occur throughout the genome, a subset of which are repaired to form reciprocal crossovers between chromosomes. Crossovers are essential to ensure balanced chromosome segregation and to create new combinations… read more here.
Keywords: spo11 prd3; chromosome axis; spo11; localisation ... See more keywords