Replication Protein A Enhances Kinetics of Uracil DNA Glycosylase on ssDNA and Across DNA Junctions: Explored with a DNA Repair Complex Produced with SpyCatcher/SpyTag Ligation
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DOI: 10.1002/cbic.202200765
Abstract: DNA repair proteins participate in extensive protein−protein interactions that promote the formation of DNA repair complexes. To understand how complex formation affects protein function during base excision repair, we used SpyCatcher/SpyTag ligation to produce a… read more here.
Keywords: dna repair; dna; ssdna; repair ... See more keywords
Orthogonal protein decoration of DNA nanostructures based on SpyCatcher-SpyTag interaction.
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DOI: 10.1039/d2cc05335g
Abstract: We present an efficient and readily applicable strategy for the covalent ligation of proteins to DNA origami by using the SpyCatcher-SpyTag (SC-ST) connector system. This approach showed orthogonality with other covalent connectors and has been… read more here.
Keywords: nanostructures based; spycatcher spytag; protein decoration; orthogonal protein ... See more keywords
Construction of Bi-Enzyme Self-Assembly Clusters Based on SpyCatcher/SpyTag for the Efficient Biosynthesis of (R)-Ethyl 2-hydroxy-4-phenylbutyrate
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DOI: 10.3390/biom13010091
Abstract: Cascade reactions catalyzed by multi-enzyme systems are important in science and industry and can be used to synthesize drugs and nutrients. In this study, two types of macromolecules of bi-enzyme self-assembly clusters (BESCs) consisting of… read more here.
Keywords: gdh; spycatcher spytag; cpcr; ethyl ... See more keywords