Improvement of Modular Protein Display Efficiency in SpyTag-Implemented Norovirus-like Particles.
Sign Up to like & getrecommendations! Published in 2022 at "Biomacromolecules"
DOI: 10.1021/acs.biomac.2c01150
Abstract: Genetic fusion and chemical conjugation are the most common approaches for displaying a foreign protein on the surface of virus-like particles (VLPs); however, these methods may negatively affect the formation and stability of VLPs. Here,… read more here.
Keywords: norovirus like; spytag; like particles; nov lps ... See more keywords
Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-019-09678-w
Abstract: Peptide tags are a key resource, introducing minimal change while enabling a consistent process to purify diverse proteins. However, peptide tags often provide minimal benefit post-purification. We previously designed SpyTag, forming an irreversible bond with… read more here.
Keywords: spytag; spy; purification spytag; purification ... See more keywords
A novel synthetic trivalent single chain variable fragment (tri-scFv) construction platform based on the SpyTag/SpyCatcher protein ligase system
Sign Up to like & getrecommendations! Published in 2018 at "BMC Biotechnology"
DOI: 10.1186/s12896-018-0466-6
Abstract: BackgroundAdvances in antibody engineering provide strategies to construct recombinant antibody-like molecules with modified pharmacokinetic properties. Multermerization is one strategy that has been used to produce antibody-like molecules with two or more antigen binding sites. Multimerization… read more here.
Keywords: spytag; scfv; anti her3; tri ... See more keywords
Tagging and catching: rapid isolation and efficient labeling of organelles using the covalent Spy-System in planta
Sign Up to like & getrecommendations! Published in 2020 at "Plant Methods"
DOI: 10.1186/s13007-020-00663-9
Abstract: Background Up-to-now, several biochemical methods have been developed to allow specific organelle isolation from plant tissues. These procedures are often time consuming, require substantial amounts of plant material, have low yield or do not result… read more here.
Keywords: spytag; system; plant; covalent ... See more keywords