Enhanced stability of a rumen-derived xylanase using SpyTag/SpyCatcher cyclization
Sign Up to like & getrecommendations! Published in 2020 at "World Journal of Microbiology and Biotechnology"
DOI: 10.1007/s11274-020-2809-4
Abstract: Microbiota from herbivore rumen is of great interest for mining glycoside hydrolases for lignocellulosic biomass biorefinement. We previously isolated a highly active but poorly thermostable xylanase (LXY) from a rumen fluid fosmid library of Hu… read more here.
Keywords: stability; spytag spycatcher; cyclization; xylanase ... See more keywords
Antigenicity and immunogenicity of HA2 and M2e influenza virus antigens conjugated to norovirus-like, VP1 capsid-based particles by the SpyTag/SpyCatcher technology.
Sign Up to like & getrecommendations! Published in 2021 at "Virology"
DOI: 10.1016/j.virol.2021.12.001
Abstract: Virus-like particles (VLPs) modified through different molecular technologies are employed as delivery vehicles or platforms for heterologous antigen display. We have recently created a norovirus (NoV) VLP platform, where two influenza antigens, the extracellular domain… read more here.
Keywords: ha2 m2e; influenza virus; virus; spytag spycatcher ... See more keywords
NMR Spectroscopic Studies Reveal the Critical Role of Isopeptide Bond in Forming the Otherwise Unstable SpyTag-SpyCatcher Mutant Complexes.
Sign Up to like & getrecommendations! Published in 2020 at "Biochemistry"
DOI: 10.1021/acs.biochem.0c00287
Abstract: The interplay between protein folding and chemical reaction has been an intriguing subject. In this contribution, we report the study of SpyTag and SpyCatcher reactive mutants using combined techniques of SDS-PAGE, LC-MS, circular dichroism, and… read more here.
Keywords: protein; spytag spycatcher; reveal critical; critical role ... See more keywords
Development of HER2-Targeting-Ligand-Modified Albumin Nanoparticles Based on the SpyTag/SpyCatcher System for Photothermal Therapy.
Sign Up to like & getrecommendations! Published in 2021 at "Biomacromolecules"
DOI: 10.1021/acs.biomac.1c00336
Abstract: The successful development of targeted nanoparticle (NP)-based therapeutics depends on the effective conjugation of targeting ligands to the NP. However, conventional methods based on chemical reactive groups such as N-hydroxysuccinimide, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, and maleimide have… read more here.
Keywords: system; targeting ligand; spytag spycatcher; her2 ... See more keywords
Fiber manipulation and post-assembly nanobody conjugation for adenoviral vector retargeting through SpyTag-SpyCatcher protein ligation
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2022.1039324
Abstract: For adenoviruses (Ads) to be optimally effective in cancer theranostics, they need to be retargeted toward target cells and lose their natural tropism. Typically, this is accomplished by either engineering fiber proteins and/or employing bispecific… read more here.
Keywords: fiber; spycatcher; spytag spycatcher; nanobody ... See more keywords
Development of SpyTag/SpyCatcher-Bacmid Expression Vector System (SpyBEVS) for Protein Bioconjugations Inside of Silkworms
Sign Up to like & getrecommendations! Published in 2019 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms20174228
Abstract: Protein conjugations at post-translational levels are known to be essential to protein stability and function. Recently, it has been proven that the split protein CnaB2 (SpyTag/SpyCatcher, ST/SC) from Streptococcus pyogenes can induce covalent conjugation rapidly… read more here.
Keywords: protein; inside silkworms; spytag spycatcher; system spybevs ... See more keywords