Establishment of a HEK293 cell line by CRISPR/Cas9-mediated luciferase knock-in to study transcriptional regulation of the human SREBP1 gene
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DOI: 10.1007/s10529-018-2608-2
Abstract: ObjectivesTo establish a HEK293 cell line with a luciferase knock-in reporter controlled by the endogenous SREBP1 promoter for investigating transcriptional regulation of the SREBP1 gene.ResultsPCR confirmed the site-specific integration of a single copy of the… read more here.
Keywords: srebp1 gene; srebp1; cell line; luciferase ... See more keywords