Identification and Mutational Analysis of Escherichia coli Sorbitol-Enhanced Glucose-Repressed srlA Promoter Expressed in LB Medium by Using Homologous Recombination and One-Round PCR Products
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DOI: 10.1007/s12033-018-0123-2
Abstract: Escherichia coli has been used for recombinant protein production for many years. However, no native E. coli promoters have been found for constitutive expression in LB medium. To obtain high-expression E. coli promoters active in… read more here.
Keywords: round pcr; one round; sorbitol enhanced; srla promoter ... See more keywords