Efficient gene knockdown in Clostridium acetobutylicum by synthetic small regulatory RNAs
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26077
Abstract: Clostridium is considered a promising microbial host for the production of valuable industrial chemicals. However, Clostridium is notorious for the difficulty of genetic manipulations, and consequently metabolic engineering. Thus, much effort has been exerted to… read more here.
Keywords: srna; acetobutylicum; clostridium; expression ... See more keywords
A novel synthetic sRNA promoting protein overexpression in cell-free systems.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology progress"
DOI: 10.1002/btpr.3324
Abstract: Bacterial small RNAs (sRNAs) that regulate gene expression have been engineered for uses in synthetic biology and metabolic engineering. Here, we designed a novel non-Hfq-dependent sRNA scaffold that makes use of a 20 nt antisense… read more here.
Keywords: cell free; novel synthetic; expression; srna ... See more keywords
Hfq chaperone brings speed dating to bacterial sRNA
Sign Up to like & getrecommendations! Published in 2018 at "Wiley Interdisciplinary Reviews: RNA"
DOI: 10.1002/wrna.1475
Abstract: Hfq is a ubiquitous, Sm‐like RNA binding protein found in most bacteria and some archaea. Hfq binds small regulatory RNAs (sRNAs), facilitates base pairing between sRNAs and their mRNA targets, and directly binds and regulates… read more here.
Keywords: hfq; chaperone brings; srna; rna ... See more keywords
Small RNA profiling in Mycobacterium tuberculosis identifies MrsI as necessary for an anticipatory iron sparing response
Sign Up to like & getrecommendations! Published in 2018 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.1718003115
Abstract: Significance This work describes the most extensive discovery and functional characterization of small regulatory RNAs (sRNAs) in Mycobacterium tuberculosis to date. We comprehensively define the sRNAs expressed in M. tuberculosis under five host-like stress conditions.… read more here.
Keywords: response; srna; tuberculosis; iron sparing ... See more keywords
Gene repression using synthetic small regulatory RNA in Methylorubrum extorquens.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of applied microbiology"
DOI: 10.1111/jam.15159
Abstract: AIM Genetic tools are a prerequisite for engineering cell factories for synthetic biology and biotechnology. Methylorubrum extorquens is an important platform for a future one-carbon (C1) bioeconomy, but its application is currently limited by the availability… read more here.
Keywords: gene repression; srna; methylorubrum extorquens; rna ... See more keywords
Probing the sRNA regulatory landscape of P. aeruginosa: post‐transcriptional control of determinants of pathogenicity and antibiotic susceptibility
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Microbiology"
DOI: 10.1111/mmi.13857
Abstract: During environmental adaptation bacteria use small regulatory RNAs (sRNAs) to repress or activate expression of a large fraction of their proteome. We extended the use of the in vivo RNA proximity ligation method toward probing… read more here.
Keywords: srna; srna regulatory; probing srna; aeruginosa post ... See more keywords
Identification and Regulatory Roles of a New Csr Small RNA from Arctic Pseudoalteromonas fuliginea BSW20308 in Temperature Responses
Sign Up to like & getrecommendations! Published in 2023 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.04094-22
Abstract: Previous research on microbial temperature adaptation has focused primarily on functional genes, with little attention paid to posttranscriptional regulation. Small RNAs, the major posttranscriptional modulators of gene expression, are greatly underexplored, especially in nonpathogenic and… read more here.
Keywords: srna; temperature; fuliginea bsw20308; gene expression ... See more keywords
Identification of oligo-adenylated small RNAs in the parasite Entamoeba and a potential role for small RNA control
Sign Up to like & getrecommendations! Published in 2020 at "BMC Genomics"
DOI: 10.1186/s12864-020-07275-6
Abstract: Background The RNA interference (RNAi) pathway is a gene regulation mechanism that utilizes small RNA (sRNA) and Argonaute (Ago) proteins to silence target genes. Our previous work identified a functional RNAi pathway in the protozoan… read more here.
Keywords: small rna; non templated; srna; parasite entamoeba ... See more keywords
A Haloarchaeal Small Regulatory RNA (sRNA) Is Essential for Rapid Adaptation to Phosphate Starvation Conditions
Sign Up to like & getrecommendations! Published in 2019 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2019.01219
Abstract: The haloarchaeon Haloferax volcanii contains nearly 2800 small non-coding RNAs (sRNAs). One intergenic sRNA, sRNA132, was chosen for a detailed characterization. A deletion mutant had a growth defect and thus underscored the importance of sRNA132.… read more here.
Keywords: srna; operon; phosphate starvation; srna132 ... See more keywords
COLR Acinetobacter baumannii sRNA Signatures: Computational Comparative Identification and Biological Targets
Sign Up to like & getrecommendations! Published in 2019 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2019.03075
Abstract: Multidrug-Resistant (MDR) and Extensively Drug Resistant (XDR) Acinetobacter baumannii (Ab) represent a serious cause of healthcare-associated infections worldwide. Currently, the available treatment options are very restricted and colistin-based therapies are last-line treatments of these infections,… read more here.
Keywords: srna signatures; srna; gene; acinetobacter baumannii ... See more keywords
A Small Non-Coding RNA Mediates Transcript Stability and Expression of Cytochrome bd Ubiquinol Oxidase Subunit I in Rickettsia conorii
Sign Up to like & getrecommendations! Published in 2023 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24044008
Abstract: Small regulatory RNAs (sRNAs) are now widely recognized for their role in the post-transcriptional regulation of bacterial virulence and growth. We have previously demonstrated the biogenesis and differential expression of several sRNAs in Rickettsia conorii… read more here.
Keywords: cydb; cytochrome ubiquinol; expression; srna ... See more keywords