Visualization and Quantification of Sortase Activity at the Single-Molecule Level via Transpeptidation-Directed Intramolecular Förster Resonance Energy Transfer.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.8b03716
Abstract: The sortase-catalyzed coupling reaction is an efficient strategy to incorporate chemically defined modifications into proteins of interest. Despite its widespread applications in protein chemistry, the conventional bulk fluorescence measurement is not sufficient to characterize sortase… read more here.
Keywords: single molecule; molecule level; sortase; activity ... See more keywords
Tideglusib and its analogs as inhibitors of Staphylococcus aureus SrtA.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of medicinal chemistry"
DOI: 10.1021/acs.jmedchem.0c00803
Abstract: Sortase A (SrtA) anchors surface proteins to the cell wall envelope, and it has attracted increasing interesting as a potential antivirulence target. Several small-molecule inhibitors for SrtA have been developed, but target validation remains largely… read more here.
Keywords: staphylococcus aureus; analogs inhibitors; srta tideglusib; inhibitors staphylococcus ... See more keywords
Quercetin impairs Streptococcus pneumoniae biofilm formation by inhibiting sortase A activity
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Cellular and Molecular Medicine"
DOI: 10.1111/jcmm.13910
Abstract: Biofilm formation mediated by sortase A (srtA) is important for bacterial colonisation and resistance to antibiotics. Thus, the inhibitor of SrtA may represent a promising agent for bacterial infection. The structure of Streptococcus pneumoniae D39… read more here.
Keywords: pneumoniae; biofilm formation; quercetin; activity ... See more keywords
Punicalagin, an Inhibitor of Sortase A, Is a Promising Therapeutic Drug to Combat Methicillin-Resistant Staphylococcus aureus Infections
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DOI: 10.1128/aac.00224-22
Abstract: Antimicrobial resistance (AMR) poses a major threat to human health globally. Staphylococcus aureus is recognized as a cause of disease worldwide, especially methicillin-resistant S. aureus (MRSA) and vancomycin-resistant S. aureus (VRSA). The enzyme sortase A… read more here.
Keywords: methicillin resistant; srta; staphylococcus aureus; sortase ... See more keywords
Uncovering Roles of Streptococcus gordonii SrtA-Processed Proteins in the Biofilm Lifestyle
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DOI: 10.1128/jb.00544-20
Abstract: S. gordonii interactions with its environment depend on the complement of cell wall proteins. A subset of these cell wall proteins requires processing by the enzyme sortase A (SrtA). The identification of SrtA-processed proteins and… read more here.
Keywords: srta; processed proteins; srta processed; gordonii ... See more keywords
Hibifolin, a Natural Sortase A Inhibitor, Attenuates the Pathogenicity of Staphylococcus aureus and Enhances the Antibacterial Activity of Cefotaxime
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DOI: 10.1128/spectrum.00950-22
Abstract: We identified hibifolin as a sortase A (SrtA) inhibitor by screening the natural compounds library, which effectively inhibited the activity of SrtA with an IC50 value of 31.20 μg/mL. Hibifolin attenuated the pathogenic behavior of Staphylococcus… read more here.
Keywords: hibifolin; srta; inhibitor; staphylococcus aureus ... See more keywords
Efficient expression of sortase A from Staphylococcus aureus in Escherichia coli and its enzymatic characterizations
Sign Up to like & getrecommendations! Published in 2017 at "Bioresources and Bioprocessing"
DOI: 10.1186/s40643-017-0143-y
Abstract: BackgroundSortase A (SrtA) is a transpeptidase found in Staphylococcus aureus, which is widely used in site-specific protein modification. However, SrtA was expressed in Escherichia coli (E. coli) in rather low level (ranging from several milligrams… read more here.
Keywords: staphylococcus aureus; escherichia coli; enzymatic characterizations; expression ... See more keywords