Transmission of poly(dT60) single‐stranded DNA through polycarbonate track‐etched ultrafiltration membranes
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Progress"
DOI: 10.1002/btpr.3315
Abstract: The objective of this study was to examine membrane filtration of a single stranded DNA (ssDNA) with 60 thymine nucleotides, and to elucidate the variables controlling its transmission across track‐etched porous membranes. Dead end filtration… read more here.
Keywords: single stranded; ssdna; size; ionic strength ... See more keywords
Replication Protein A Enhances Kinetics of Uracil DNA Glycosylase on ssDNA and Across DNA Junctions: Explored with a DNA Repair Complex Produced with SpyCatcher/SpyTag Ligation
Sign Up to like & getrecommendations! Published in 2023 at "ChemBioChem"
DOI: 10.1002/cbic.202200765
Abstract: DNA repair proteins participate in extensive protein−protein interactions that promote the formation of DNA repair complexes. To understand how complex formation affects protein function during base excision repair, we used SpyCatcher/SpyTag ligation to produce a… read more here.
Keywords: dna repair; dna; ssdna; repair ... See more keywords
An Efficient Bead‐captured Denaturation Method for Preparing Long Single‐stranded DNA
Sign Up to like & getrecommendations! Published in 2017 at "Journal of The Chinese Chemical Society"
DOI: 10.1002/jccs.201700142
Abstract: Previous methods to prepare single-stranded DNA (ssDNA) substrates are limited to short DNA lengths and inefficient. We have developed an efficient and rapid method to prepare long ssDNA substrates (up to 4000 nt) based on the… read more here.
Keywords: single stranded; dna; ssdna; bead captured ... See more keywords
Chaperoning RPA during DNA metabolism
Sign Up to like & getrecommendations! Published in 2019 at "Current Genetics"
DOI: 10.1007/s00294-019-00945-3
Abstract: Single-stranded DNA (ssDNA) is widely generated during DNA metabolisms including DNA replication, repair and recombination and is susceptible to digestion by nucleases and secondary structure formation. It is vital for DNA metabolism and genome stability… read more here.
Keywords: dna metabolism; dna; ssdna; replication ... See more keywords
Manufacturing of a novel double-function ssDNA aptamer for sensitive diagnosis and efficient neutralization of SEA.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2018.02.017
Abstract: Staphylococcal enterotoxin A (SEA) is an enterotoxin produced mainly by Staphylococcus aureus. In recent years, it has become the most prevalent compound for staphylococcal food poisoning (SFP) around the world. In this study, we isolate… read more here.
Keywords: sea; ssdna; neutralization sea; function ... See more keywords
Complexed crystal structure of SSB reveals a novel single-stranded DNA binding mode (SSB)3:1: Phe60 is not crucial for defining binding paths.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2019.10.036
Abstract: Single-stranded DNA-binding protein (SSB) is essential to cells as it participates in DNA metabolic processes, such as DNA replication, repair, and recombination. Escherichia coli SSB (EcSSB) tetramer cooperatively binds and wraps ssDNA in two major… read more here.
Keywords: mode; ssb; ssdna; dna binding ... See more keywords
The meiosis-specific MEIOB-SPATA22 complex cooperates with RPA to form a compacted mixed MEIOB/SPATA22/RPA/ssDNA complex.
Sign Up to like & getrecommendations! Published in 2021 at "DNA repair"
DOI: 10.1016/j.dnarep.2021.103097
Abstract: During meiosis, programmed double-strand breaks are repaired by homologous recombination (HR) to form crossovers that are essential to homologous chromosome segregation. Single-stranded DNA (ssDNA) containing intermediates are key features of HR, which must be highly… read more here.
Keywords: meiob spata22; spata22; ssdna; spata22 rpa ... See more keywords
Aptamer-Target Recognition-Promoted Ratiometric Electrochemical Strategy for Evaluating the Microcystin-LR Residue in Fish without Interferences.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.1c06476
Abstract: Given the significance of food safety, it is highly urgent to develop a sensitive yet reliable sensor for the practical analysis of algal toxins. As most of the developed sensors are disturbed by interfering substances… read more here.
Keywords: ratiometric electrochemical; recognition; ssdna; aptamer target ... See more keywords
Ultralarge Modulation of Fluorescence by Neuromodulators in Carbon Nanotubes Functionalized with Self-Assembled Oligonucleotide Rings.
Sign Up to like & getrecommendations! Published in 2018 at "Nano letters"
DOI: 10.1021/acs.nanolett.8b02937
Abstract: Noncovalent interactions between single-stranded DNA (ssDNA) oligonucleotides and single wall carbon nanotubes (SWNTs) have provided a unique class of tunable chemistries for a variety of applications. However, mechanistic insight into both the photophysical and intermolecular… read more here.
Keywords: modulation; fluorescence; ultralarge modulation; ssdna ... See more keywords
Ionic Liquid Decelerates Single-Stranded DNA Transport through Molybdenum Disulfide Nanopores.
Sign Up to like & getrecommendations! Published in 2022 at "ACS applied materials & interfaces"
DOI: 10.1021/acsami.2c03335
Abstract: Nanopores in two-dimensional (2D) materials have emerged to offer in principle necessary spatial resolution for high-throughput DNA sequencing. However, their fidelity is severely limited by the fast DNA translocation. A recent experiment indicates that introducing… read more here.
Keywords: ionic liquid; ssdna; dna translocation; dna ... See more keywords
Sub-nanomolar FRET-based DNA Assay Using Thermally Stable Phosphorothioated DNA-functionalized Quantum Dots.
Sign Up to like & getrecommendations! Published in 2019 at "ACS applied materials & interfaces"
DOI: 10.1021/acsami.9b07717
Abstract: Quantum dots (QDs) can serve as an attractive Förster resonance energy transfer (FRET) donor for DNA assay due to their excellent optical properties. However, the specificity and sensitivity of QD-based FRET analysis are prominently reduced… read more here.
Keywords: nanomolar fret; dna; ssdna; sub nanomolar ... See more keywords