Effects of intragenomic polymorphism in the SSU rRNA gene on estimating marine microeukaryotic diversity: A test for ciliates using single‐cell high‐throughput DNA sequencing
Sign Up to like & getrecommendations! Published in 2019 at "Limnology and Oceanography: Methods"
DOI: 10.1002/lom3.10330
Abstract: Diversity assessments using the small subunit (SSU) rRNA gene allow for rapid assessments of microeukaryotic communities. Yet, for microeukaryotes, only limited information is available on the extent to which sequence variations within individual genomes exist… read more here.
Keywords: rrna gene; diversity; ssu rrna; cell ... See more keywords
Morphological and molecular characterization of Eustrongylides excisus larvae (Nematoda: Dioctophymatidae) in Sander lucioperca (L.) from Northern Turkey.
Sign Up to like & getrecommendations! Published in 2021 at "Parasitology research"
DOI: 10.1007/s00436-021-07187-8
Abstract: The genus Eustrongylides Jägerskiöld, 1909 includes parasitic nematodes (Dioctophymatidae) affecting various fish species and piscivorous birds of freshwater ecosystems. Currently, there is little information on the molecular characterization of E. excisus based on nuclear ribosomal… read more here.
Keywords: molecular characterization; northern turkey; ssu rrna; lucioperca northern ... See more keywords
Retrieval of a million high-quality, full-length microbial 16S and 18S rRNA gene sequences without primer bias
Sign Up to like & getrecommendations! Published in 2018 at "Nature Biotechnology"
DOI: 10.1038/nbt.4045
Abstract: Small subunit ribosomal RNA (SSU rRNA) genes, 16S in bacteria and 18S in eukaryotes, have been the standard phylogenetic markers used to characterize microbial diversity and evolution for decades. However, the reference databases of full-length… read more here.
Keywords: primer bias; full length; ssu rrna; rrna ... See more keywords
Migrating SSU rRNA gene surveys to the metagenomics era.
Sign Up to like & getrecommendations! Published in 2017 at "Environmental microbiology reports"
DOI: 10.1111/1758-2229.12493
Abstract: Having just celebrated a decade of “next-generation sequencing”, or high-throughput sequencing, it is easy to overlook how revolutionary these technological innovations were for microbial ecology. Before the advent of high-throughput sequencing, environmental microbiologists combined fingerprinting… read more here.
Keywords: gene surveys; migrating ssu; ssu rrna; rrna gene ... See more keywords
phyloFlash: Rapid Small-Subunit rRNA Profiling and Targeted Assembly from Metagenomes
Sign Up to like & getrecommendations! Published in 2020 at "mSystems"
DOI: 10.1128/msystems.00920-20
Abstract: To track organisms across all domains of life, the SSU rRNA gene is the gold standard. Many environmental microbes are known only from high-throughput sequence data, but the SSU rRNA gene, the key to visualization… read more here.
Keywords: ssu rrna; targeted assembly; rrna gene; rrna ... See more keywords
From Argentine Abyssal Plain to farmed turbot in Spain: A ubiquitous amoeba species Vannella robusta sp. nov. (Amoebozoa, Vannellida).
Sign Up to like & getrecommendations! Published in 2022 at "European journal of protistology"
DOI: 10.2139/ssrn.4128769
Abstract: A strain with the characters of the genus Vannella was isolated from the water layer immediately above the deep-sea sediment collected in the south-western Atlantic Ocean, ca. 4.6 km deep. Small-subunit ribosomal RNA (SSU rRNA) and… read more here.
Keywords: gene sequences; ssu rrna; argentine abyssal; abyssal plain ... See more keywords
Detection and identification of fungi in the lower airway of children with and without cystic fibrosis
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2023.1119703
Abstract: Introduction Airway infection and inflammation lead to the progression of obstructive lung disease in persons with cystic fibrosis (PWCF). However, cystic fibrosis (CF) fungal communities, known drivers of CF pathophysiology, remain poorly understood due to… read more here.
Keywords: children without; rrna sequencing; ssu rrna; lower airway ... See more keywords
Comparison of Three Real-Time PCR Assays Targeting the SSU rRNA Gene, the COWP Gene and the DnaJ-Like Protein Gene for the Diagnosis of Cryptosporidium spp. in Stool Samples
Sign Up to like & getrecommendations! Published in 2021 at "Pathogens"
DOI: 10.3390/pathogens10091131
Abstract: As qualified microscopy of enteric parasitoses as defined by high diagnostic accuracy is difficult to maintain in non-endemic areas due to scarce opportunities for practicing with positive sample materials, molecular diagnostic options provide less investigator-dependent… read more here.
Keywords: ssu rrna; cryptosporidium spp; pcr; rrna gene ... See more keywords