Polyelectrolytes Tailored Enzyme Cascades with Enhanced Stability and Activity for One‐pot Synthesis
Sign Up to like & getrecommendations! Published in 2018 at "ChemCatChem"
DOI: 10.1002/cctc.201801532
Abstract: Cascade enzymes from the same origin or different resources often differ in their pH windows of stability and activity, thus impeding one‐pot enzyme cascade reactions. Here we present a method to assemble enzyme cascades using… read more here.
Keywords: stability activity; activity; one pot; cascade ... See more keywords
Molecular level insight into the effect of triethyloctylammonium bromide on the structure, thermal stability, and activity of Bovine serum albumin.
Sign Up to like & getrecommendations! Published in 2018 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2017.08.157
Abstract: Understanding the interactions between protein and ionic liquids (IL) is vital in order to avail the ILs in biological applications. In this study, we have investigated the influence of triethyloctylammonium bromide on the structure, stability,… read more here.
Keywords: stability activity; bromide structure; triethyloctylammonium bromide; activity bovine ... See more keywords
Glycine-rich loop encompassing active site at interface of hexameric M. tuberculosis Eis protein contributes to its structural stability and activity.
Sign Up to like & getrecommendations! Published in 2018 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2017.12.058
Abstract: RvEis is a crucial thermostable hexameric aminoglycoside acetyltransferase of Mycobacterium tuberculosis, overexpression of which confers Kanamycin resistance in clinical strains. The thermostability associated with hexameric RvEis is important for the enhanced intracellular survival of mycobacteria.… read more here.
Keywords: stability activity; glycine rich; tuberculosis; stability ... See more keywords
Crowded milieu tuning the stability and activity of stem bromelain.
Sign Up to like & getrecommendations! Published in 2018 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2017.12.060
Abstract: Proteins in vivo are under an extremely crowded environment because of the presence of bulky and large biological macromolecules (known as crowders). These crowders affect the proper functioning and structure of proteins in a cell.… read more here.
Keywords: protein; stability activity; stem bromelain; stability ... See more keywords
Natural and Synthetic Suppressor Mutations Defy Stability–Activity Tradeoffs
Sign Up to like & getrecommendations! Published in 2022 at "Biochemistry"
DOI: 10.1021/acs.biochem.1c00805
Abstract: Thermodynamic stability represents one important constraint on protein evolution, but the molecular basis for how mutations that change stability impact fitness remains unclear. Here, we demonstrate that a prevalent global suppressor mutation in TEM β-lactamase,… read more here.
Keywords: suppressor; natural synthetic; stability; stability activity ... See more keywords
Isothermal Compressibility Perturbation as a Protein Design Principle for T1 Lipase Stability-Activity Trade-Off Counteracting.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.3c01684
Abstract: Given the widely existing stability-activity trade-off in enzyme evolution, it is still a goal to obtain enzymes embracing both high activity and stability. Herein, we employed an isothermal compressibility (βT) perturbation engineering (ICPE) strategy to… read more here.
Keywords: isothermal compressibility; activity; stability; stability activity ... See more keywords
Beyond Stability Constraints: A Biophysical Model of Enzyme Evolution with Selection on Stability and Activity
Sign Up to like & getrecommendations! Published in 2019 at "Molecular Biology and Evolution"
DOI: 10.1093/molbev/msy244
Abstract: The rate of evolution varies among sites within proteins. In enzymes, two rate gradients are observed: rate decreases with increasing local packing and it increases with increasing distance from catalytic residues. The rate-packing gradient would… read more here.
Keywords: stability constraints; evolution; stability activity; rate ... See more keywords
SIRT7 modulates the stability and activity of the renal K‐Cl cotransporter KCC4 through deacetylation
Sign Up to like & getrecommendations! Published in 2021 at "EMBO reports"
DOI: 10.15252/embr.202050766
Abstract: SIRT7 is a NAD+‐dependent deacetylase that controls important aspects of metabolism, cancer, and bone formation. However, the molecular targets and functions of SIRT7 in the kidney are currently unknown. In silico analysis of kidney transcripts… read more here.
Keywords: stability activity; activity; cotransporter kcc4; sirt7 ... See more keywords
Improvement of the Stability and Activity of an LPMO Through Rational Disulfide Bonds Design
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2021.815990
Abstract: Lytic polysaccharide monooxygenases (LPMOs) oxidatively break down the glycosidic bonds of crystalline polysaccharides, significantly improving the saccharification efficiency of recalcitrant biomass, and have broad application prospects in industry. To meet the needs of industrial applications,… read more here.
Keywords: bonds design; rational disulfide; activity; stability ... See more keywords
Single Residue Substitution at N-Terminal Affects Temperature Stability and Activity of L2 Lipase
Sign Up to like & getrecommendations! Published in 2020 at "Molecules"
DOI: 10.3390/molecules25153433
Abstract: Rational design is widely employed in protein engineering to tailor wild-type enzymes for industrial applications. The typical target region for mutation is a functional region like the catalytic site to improve stability and activity. However,… read more here.
Keywords: temperature stability; stability activity; lipase; stability ... See more keywords