Hot CoFi Blot: A High-Throughput Colony-Based Screen for Identifying More Thermally Stable Protein Variants.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9624-7_14
Abstract: Highly soluble and stable proteins are desirable for many different applications, from basic science to reaching a cancer patient in the form of a biological drug. For X-ray crystallography-where production of a protein crystal might… read more here.
Keywords: cofi blot; stable protein; hot cofi; thermally stable ... See more keywords
A High-Throughput System for Transient and Stable Protein Production in Mammalian Cells.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9624-7_5
Abstract: Recombinant protein expression and purification is an essential component of biomedical research and drug discovery. Advances in automation and laboratory robotics have enabled the development of highly parallel and rapid processes for cell culture and… read more here.
Keywords: protein; transient stable; production; protein production ... See more keywords
Recipes for Inducing Cold Denaturation in an Otherwise Stable Protein.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.1c13355
Abstract: Although cold denaturation is a fundamental phenomenon common to all proteins, it can only be observed in a handful of cases where it occurs at temperatures above the freezing point of water. Understanding the mechanisms… read more here.
Keywords: denaturation; stable protein; cold denaturation; denaturation otherwise ... See more keywords