Does Cas9-Catalyzed DNA Cleavage Generate Blunt Ends or Staggered Ends? Insight from Molecular Dynamic Simulations
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DOI: 10.1016/j.bpj.2016.11.300
Abstract: The CRISPR-associated endonuclease Cas9 from Streptococcus pyogenes (spCas9) along with a single guide RNA (sgRNA) has merged as a versatile toolbox for genome editing. Despite recent advances in the mechanistic studies on spCas9-sgRNA-mediated target double-stranded… read more here.
Keywords: dna; cleavage; staggered ends; blunt ends ... See more keywords