Disrupting Interactions Between β‐Catenin and Activating TCFs Reconstitutes Ground State Pluripotency in Mouse Embryonic Stem Cells
Sign Up to like & getrecommendations! Published in 2017 at "STEM CELLS"
DOI: 10.1002/stem.2647
Abstract: The 2i‐media, composed of two small molecule inhibitors (PD0325901 and CHIR99021) against MEK and GSK3‐kinases, respectively, is known to establish naïve ground state pluripotency in mouse embryonic stem cells (mESCs). These inhibitors block MEK‐mediated differentiation,… read more here.
Keywords: state; stem cells; pluripotency; state pluripotency ... See more keywords
Activation of transcription factor circuity in 2i-induced ground state pluripotency is independent of repressive global epigenetic landscapes
Sign Up to like & getrecommendations! Published in 2020 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkaa529
Abstract: Abstract Mouse embryonic stem cells (mESCs) cultured with MEK/ERK and GSK3β (2i) inhibitors transition to ground state pluripotency. Gene expression changes, redistribution of histone H3K27me3 profiles and global DNA hypomethylation are hallmarks of 2i exposure,… read more here.
Keywords: state pluripotency; ground state; ground; transcription factor ... See more keywords