A straightforward STED-background corrected fitting model for unbiased STED-FCS analyses.
Sign Up to like & getrecommendations! Published in 2018 at "Methods"
DOI: 10.1016/j.ymeth.2018.02.010
Abstract: Combining stimulated emission depletion and fluorescence correlation spectroscopy (STED-FCS) provides a powerful and sensitive tool for studying the molecular dynamics in live cells with high spatio-temporal resolution. STED-FCS gives access to molecular diffusion characteristic at… read more here.
Keywords: sted background; model; fitting model; sted fcs ... See more keywords
Measurement of nanoscale three-dimensional diffusion in the interior of living cells by STED-FCS
Sign Up to like & getrecommendations! Published in 2017 at "Nature Communications"
DOI: 10.1038/s41467-017-00117-2
Abstract: The observation of molecular diffusion at different spatial scales, and in particular below the optical diffraction limit ( read more here.
Keywords: three dimensional; diffusion; diffraction; spectroscopy ... See more keywords