Cleavable Linker Incorporation into a Synthetic Dye‐Nanobody‐Fluorescent Protein Assembly: FRET, FLIM and STED Microscopy
Sign Up to like & getrecommendations! Published in 2022 at "Chembiochem"
DOI: 10.1002/cbic.202200395
Abstract: A bright and photostable fluorescent dye with a disulfide (S−S) linker and maleimide group (Rho594‐S2‐mal), as cleavable and reactive sites, was synthesized and conjugated with anti‐GFP nanobodies (NB). The binding of EGFP (FRET donor) with… read more here.
Keywords: sted microscopy; microscopy; linker; dye ... See more keywords
Sample Preparation for Multicolor STED Microscopy.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2051-9_15
Abstract: Stimulated emission depletion (STED) microscopy is one of the optical superresolution microscopy (SRM) techniques, more recently also referred to as nanoscopy, that have risen to popularity among biologists during the past decade. These techniques keep… read more here.
Keywords: sample preparation; srm; microscopy; sted microscopy ... See more keywords
Multi-label in vivo STED microscopy by parallelized switching of reversibly switchable fluorescent proteins.
Sign Up to like & getrecommendations! Published in 2021 at "Cell reports"
DOI: 10.1016/j.celrep.2021.109192
Abstract: Despite the tremendous success of super-resolution microscopy, multi-color in vivo applications are still rare. Here we present live-cell multi-label STED microscopy in vivo and in vitro by combining spectrally separated excitation and detection with temporal sequential imaging of… read more here.
Keywords: reversibly switchable; vivo; microscopy; multi label ... See more keywords
Super-resolution STED microscopy in live brain tissue
Sign Up to like & getrecommendations! Published in 2021 at "Neurobiology of Disease"
DOI: 10.1016/j.nbd.2021.105420
Abstract: STED microscopy is one of several fluorescence microscopy techniques that permit imaging at higher spatial resolution than what the diffraction-limit of light dictates. STED imaging is unique among these super-resolution modalities in being a beam-scanning… read more here.
Keywords: brain tissue; resolution; sted microscopy; microscopy ... See more keywords
Assessing photodamage in live-cell STED microscopy
Sign Up to like & getrecommendations! Published in 2018 at "Nature Methods"
DOI: 10.1038/s41592-018-0145-5
Abstract: To the Editor — The recent breakthroughs in the development of optical nanoscopy have provided unprecedented views of the inner workings of cells. Stimulated emission depletion (STED) microscopy, in particular, allows real-time observation of living… read more here.
Keywords: photodamage; microscopy; cell; sted microscopy ... See more keywords
Self-healing dyes for super-resolution fluorescence microscopy
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Physics D: Applied Physics"
DOI: 10.1088/1361-6463/aae752
Abstract: In recent years, optical microscopy techniques have emerged that allow optical imaging at unprecedented resolution beyond the diffraction limit. These techniques exploit photostabilizing buffers to enable photoswitching and/or the enhancement of fluorophore brightness and stability.… read more here.
Keywords: resolution; microscopy; super resolution; self healing ... See more keywords
Investigation on achieving super-resolution by solid immersion lens based STED microscopy.
Sign Up to like & getrecommendations! Published in 2017 at "Optics express"
DOI: 10.1364/oe.25.016629
Abstract: The feasibility of stimulated emission depletion (STED) microscopy using a solid immersion lens was investigated. First, the theoretical feasibility of the considered system is discussed based on a vectorial field algorithm that uses a stratified… read more here.
Keywords: resolution; microscopy; immersion lens; solid immersion ... See more keywords
STED microscopy reveals in-situ photoluminescence properties of single nanostructures in densely perovskite thin films.
Sign Up to like & getrecommendations! Published in 2021 at "Optics express"
DOI: 10.1364/oe.442345
Abstract: All-inorganic perovskite nanomaterials have attracted much attention recently due to their prominent optical performance and potential application for optoelectronic devices. The carriers dynamics of all-inorganic perovskites has been the research focus because the understanding of… read more here.
Keywords: microscopy reveals; microscopy; situ photoluminescence; dynamics process ... See more keywords
Photoswitching the injected energy flux via core-sensitized energy migration upconversion for emission-varying STED microscopy.
Sign Up to like & getrecommendations! Published in 2022 at "Optics letters"
DOI: 10.1364/ol.464515
Abstract: Stimulated emission depletion (STED) microscopy achieved with lanthanide-doped upconversion nanoparticles (UCNPs) exhibits many outstanding advantages such as low-power illumination, near-infrared (NIR) excitation, and high photostability. However, the available types of UCNP-STED probes are very limited… read more here.
Keywords: sted microscopy; energy migration; energy; upconversion ... See more keywords
Visualization of early influenza A virus trafficking in human dendritic cells using STED microscopy
Sign Up to like & getrecommendations! Published in 2017 at "PLoS ONE"
DOI: 10.1371/journal.pone.0177920
Abstract: Influenza A viruses (IAV) primarily target respiratory epithelial cells, but can also replicate in immune cells, including human dendritic cells (DCs). Super-resolution microscopy provides a novel method of visualizing viral trafficking by overcoming the resolution… read more here.
Keywords: trafficking human; microscopy; influenza; dendritic cells ... See more keywords