New coumarin- and phenoxazine-based fluorescent probes for live-cell STED nanoscopy
Sign Up to like & getrecommendations! Published in 2019 at "European Biophysics Journal"
DOI: 10.1007/s00249-019-01354-7
Abstract: The potential of live-cell stimulated emission depletion (STED) nanoscopy has not yet been fully exploited. Currently, the main limitation is the small number of fluorophores and probes that can sustain high light intensity/high dose employed… read more here.
Keywords: live cell; tested probes; cell sted; sted nanoscopy ... See more keywords
STED Nanoscopy Imaging of Cellular Lipid Droplets Employing a Superior Organic Fluorescent Probe.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c03474
Abstract: Lipid droplets (LDs) are spherical organelles that participate in numerous biological processes. In order to visualize LDs on the nanoscale, nanoscopy fluorescence imaging is considered as the most attractive technique but is substantially limited by… read more here.
Keywords: fluorescent probe; nanoscopy imaging; probe; lipid droplets ... See more keywords
Organic Nanoparticles-Assisted Low-Power STED Nanoscopy.
Sign Up to like & getrecommendations! Published in 2021 at "Nano letters"
DOI: 10.1021/acs.nanolett.1c00161
Abstract: Stimulated emission depletion (STED) nanoscopy plays a key role in achieving sub-50 nm high spatial resolution for subcellular live-cell imaging. To avoid re-excitation, the STED wavelength has to be tuned at the red tail of… read more here.
Keywords: resolution; power; sted nanoscopy; power sted ... See more keywords
Near-infrared STED nanoscopy with an engineered bacterial phytochrome
Sign Up to like & getrecommendations! Published in 2018 at "Nature Communications"
DOI: 10.1038/s41467-018-07246-2
Abstract: The near infrared (NIR) optical window between the cutoff for hemoglobin absorption at 650 nm and the onset of increased water absorption at 900 nm is an attractive, yet largely unexplored, spectral regime for diffraction-unlimited super-resolution fluorescence… read more here.
Keywords: nanoscopy engineered; microscopy; near infrared; infrared sted ... See more keywords
Mitochondrial dynamics quantitatively revealed by STED nanoscopy with an enhanced squaraine variant probe
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-17546-1
Abstract: Mitochondria play a critical role in generating energy to support the entire lifecycle of biological cells, yet it is still unclear how their morphological structures evolve to regulate their functionality. Conventional fluorescence microscopy can only… read more here.
Keywords: squaraine variant; resolution; variant; variant probe ... See more keywords
Live-cell STED nanoscopy of mitochondrial cristae
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-48838-2
Abstract: Mitochondria are highly dynamic organelles that exhibit a complex inner architecture. They exhibit a smooth outer membrane and a highly convoluted inner membrane that forms invaginations called cristae. Imaging cristae in living cells poses a… read more here.
Keywords: live cell; cristae; nanoscopy mitochondrial; cell sted ... See more keywords