Detection of piRNA‐54265 in human serum: evidence and significance
Sign Up to like & getrecommendations! Published in 2022 at "Cancer Communications"
DOI: 10.1002/cac2.12381
Abstract: Dear Editor, An increasing number of studies have shown that PIWIinteracting RNAs (piRNAs) are aberrantly expressed in many types of human cancer and involved in regulating the malignant progression, piRNAs would be expected to serve… read more here.
Keywords: pirna 54265; pir 54265; stem loop; human serum ... See more keywords
RT-qPCR with chimeric dU stem-loop primer is efficient for the detection of bacterial small RNAs
Sign Up to like & getrecommendations! Published in 2017 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-017-8181-0
Abstract: Small non-coding RNAs are considered be involved in the regulation of multiple cellular processes. Quantitative reverse transcription PCR (RT-qPCR) is widely used in the detection of eukaryotic microRNA, and the stem-loop primers can improve the… read more here.
Keywords: detection; loop primer; stem loop; stem ... See more keywords
A novel insight in favor of structure–function relationship for 16S rRNA
Sign Up to like & getrecommendations! Published in 2018 at "Molecular Biology Reports"
DOI: 10.1007/s11033-018-4274-9
Abstract: Sequences in the stem-loop part of 16S ribosomal RNA (rRNA) are considered to be crucial for predicting antibiotic resistance. Mutant sequences have been reported to be helpful in the prediction of spectinomycin resistance. It is… read more here.
Keywords: rrna; novel insight; 16s rrna; stem loop ... See more keywords
SAFB2 Enables the Processing of Suboptimal Stem-Loop Structures in Clustered Primary miRNA Transcripts.
Sign Up to like & getrecommendations! Published in 2020 at "Molecular cell"
DOI: 10.1016/j.molcel.2020.05.011
Abstract: Many microRNAs (miRNAs) are generated from primary transcripts containing multiple clustered stem-loop structures that are thought to be recognized and cleaved by the Microprocessor complex as independent units. Here, we uncover an unexpected mode of… read more here.
Keywords: mirna; processing suboptimal; loop structures; stem loop ... See more keywords
Structural Stability of the Anticodon Stem Loop Domains of the Unmodified Yeast and Escherichia coli tRNAPhe: Differing Views from Different Force Fields
Sign Up to like & getrecommendations! Published in 2019 at "ACS Omega"
DOI: 10.1021/acsomega.8b02383
Abstract: Unmodified tRNAs provide essential insights into the structural and functional role of the extensive and diverse post-transcriptional modifications that naturally occur in tRNAs. The X-ray crystal structure of the completely unmodified Escherichia coli tRNAPhe showed… read more here.
Keywords: coli trnaphe; anticodon stem; stem loop; escherichia coli ... See more keywords
Evaluation of Rationally Designed Label-free Stem-loop DNA Probe Opening in the Presence of miR-21 by Circular Dichroism and Fluorescence Techniques
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-60157-5
Abstract: The characteristic features of stem-loop structured probes make them robust tools to detect targets with high sensitivity and selectivity. The basis of the hairpin based sensors operation is a conformational change that occurs upon hybridization… read more here.
Keywords: probe; designed label; target; spectroscopy ... See more keywords
Synthesis and properties of the anticodon stem-loop of human mitochondrial tRNAMet containing the disease-related G or m1G nucleosides at position 37.
Sign Up to like & getrecommendations! Published in 2021 at "Chemical communications"
DOI: 10.1039/d1cc05215b
Abstract: A single point mutation (A4435G) in the human mitochondrial tRNAMet (hmt-tRNAMet) gene causes severe mitochondrial disorders associated with hypertension, type 2 diabetes and LHON. This mutation leads to the exchange of A37 in the anticodon… read more here.
Keywords: mitochondrial trnamet; anticodon stem; human mitochondrial; stem loop ... See more keywords
Targeting stem-loop 1 of the SARS-CoV-2 5′ UTR to suppress viral translation and Nsp1 evasion
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2117198119
Abstract: Significance The COVID-19 pandemic and the ever-evolving variants of SARS-CoV-2 are taking a toll on human health. Despite the successful rollout of vaccines, effective therapies are still urgently needed. Our studies here showing that Nsp1… read more here.
Keywords: sars cov; stem loop; viral translation; cov utr ... See more keywords
Variance in translational fidelity of different bacterial species is affected by pseudouridines in the tRNA anticodon stem-loop
Sign Up to like & getrecommendations! Published in 2022 at "RNA Biology"
DOI: 10.1080/15476286.2022.2121447
Abstract: ABSTRACT Delicate variances in the translational machinery affect how efficiently different organisms approach protein synthesis. Determining the scale of this effect, however, requires knowledge on the differences of mistranslation levels. Here, we used a dual-luciferase… read more here.
Keywords: stem loop; fidelity; anticodon stem; variance translational ... See more keywords
A predicted stem-loop in coat protein-coding sequencing of tobacco vein banding mosaic virus is required for efficient replication.
Sign Up to like & getrecommendations! Published in 2021 at "Phytopathology"
DOI: 10.1094/phyto-10-20-0463-r
Abstract: Potyviral Coat protein (CP) is involved in the replication and movement of potyviruses. However, little information is available on the roles of CP-coding sequence in potyviral infection. Here, we introduced synonymous substitutions to the codon… read more here.
Keywords: tvbmv; tobacco vein; stem loop; replication ... See more keywords
Enhancement of Regnase-1 expression with stem loop–targeting antisense oligonucleotides alleviates inflammatory diseases
Sign Up to like & getrecommendations! Published in 2022 at "Science Translational Medicine"
DOI: 10.1126/scitranslmed.abo2137
Abstract: Regnase-1 is an ribonuclease that plays essential roles in restricting inflammation through degrading messenger RNAs (mRNAs) involved in immune reactions via the recognition of stem-loop (SL) structures in the 3′ untranslated regions (3′UTRs). Dysregulated expression… read more here.
Keywords: regnase expression; stem loop; expression; regnase ... See more keywords