Identification of key residues modulating the stereoselectivity of nitrile hydratase toward rac‐mandelonitrile by semi‐rational engineering
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26484
Abstract: Optically pure compounds are important in the synthesis of fine chemicals. Using directed evolution of enzymes to obtain biocatalysts that can selectively produce high‐value chiral chemicals is often time‐, money‐, and resource‐intensive; traditional semi‐rational designs… read more here.
Keywords: semi rational; stereoselectivity; man; nitrile hydratase ... See more keywords
A High‐Throughput Visual Screen for the Directed Evolution of Cβ‐stereoselectivity of L‐threonine Aldolase
Sign Up to like & getrecommendations! Published in 2024 at "ChemBioChem"
DOI: 10.1002/cbic.202400637
Abstract: L‐Threonine aldolase (L‐TA) is a pyridoxal phosphate‐dependent enzyme that catalyzes the reversible condensation of glycine and aldehydes to form β‐hydroxy‐α‐amino acids. The combination of directed evolution and efficient high‐throughput screening methods is an effective strategy… read more here.
Keywords: high throughput; directed evolution; throughput; stereoselectivity ... See more keywords
Origin of Regio‐ and Stereoselectivity in the NHC‐catalyzed Reaction of Alkyl Pyridinium with Aliphatic Enal
Sign Up to like & getrecommendations! Published in 2019 at "ChemCatChem"
DOI: 10.1002/cctc.201901965
Abstract: The mechanisms of N‐heterocyclic carbene (NHC)‐catalyzed dearomatization reaction of alkyl pyridinium have been intensively studied using the density functional theory (DFT) method. The chemoselectivity and stereoselectivity were both analyzed based on the established mechanisms. The… read more here.
Keywords: nhc catalyzed; alkyl pyridinium; reaction alkyl; stereoselectivity ... See more keywords
Systemic stereoselectivity study of bromothalonil: stereoselective bioactivity, toxicity, and degradation in vegetables and soil.
Sign Up to like & getrecommendations! Published in 2019 at "Pest management science"
DOI: 10.1002/ps.5711
Abstract: BACKGROUND The chiral pesticide bromothalonil is widely used to control anthracnose disease on fruits and vegetables in China. The pesticidal activity, ecotoxicological effects and environmental behavior of bromothalonil and its enantiomers are still unclear. RESULTS… read more here.
Keywords: degradation; bromothalonil; bromothalonil stereoselective; stereoselectivity ... See more keywords
Characterization of CyrI, the hydroxylase involved in the last step of cylindrospermopsin biosynthesis: Binding studies, site-directed mutagenesis and stereoselectivity.
Sign Up to like & getrecommendations! Published in 2018 at "Archives of biochemistry and biophysics"
DOI: 10.1016/j.abb.2018.04.007
Abstract: Cylindrospermopsin, a cytotoxin from cyanobacteria, is biosynthesized by a complex pathway, which involves CyrI, an iron and 2-oxoglutarate dependent hydroxylase that transforms 7-deoxy-cylindrospermopsin into cylindrospermopsin and its epimer, 7-epi-cylindrospermopsin, in the last step. The activity… read more here.
Keywords: cyri; hydroxylase; cylindrospermopsin; stereoselectivity ... See more keywords
Design and Application of New Pyridine-Derived Chiral Ligands in Asymmetric Catalysis.
Sign Up to like & getrecommendations! Published in 2024 at "Accounts of chemical research"
DOI: 10.1021/acs.accounts.3c00808
Abstract: ConspectusThe innovation of chiral ligands has been crucial for the asymmetric synthesis of functional molecules, as demonstrated by several types of widely applied "privileged" ligands. In this context, chiral pyridine-derived ligands, by far some of… read more here.
Keywords: pyridine derived; pyridine; chiral ligands; cpu ... See more keywords
Mechanism, Stereoselectivity, and Role of O2 in Aza-Diels-Alder Reactions Catalyzed by Dinuclear Molybdenum Complexes: A Theoretical Study.
Sign Up to like & getrecommendations! Published in 2022 at "Inorganic chemistry"
DOI: 10.1021/acs.inorgchem.2c00035
Abstract: The aza-Diels-Alder-type reaction between imines and functionalized alkenes is one of the most versatile approaches to obtain piperidine derivatives. When using the Lewis acid [Mo2(OAc)4] (CAT) as a catalyst, it was found that the activation… read more here.
Keywords: aza diels; stereoselectivity; reaction; diels alder ... See more keywords
Investigating Enzyme Active-Site Geometry and Stereoselectivity in an Undergraduate Biochemistry Lab
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Chemical Education"
DOI: 10.1021/acs.jchemed.6b00772
Abstract: The three-dimensional nature of interactions between enzymes and their substrates often leads to exacting spatial binding orientations and stereoselectivity in chemical catalysis. Dehydrogenases that use NAD+ as a redox cofactor tend to show stereospecificity in… read more here.
Keywords: biochemistry; investigating enzyme; stereoselectivity; geometry ... See more keywords
Computation-Aided Engineering of Cytochrome P450 for the Production of Pravastatin
Sign Up to like & getrecommendations! Published in 2022 at "ACS Catalysis"
DOI: 10.1021/acscatal.2c03974
Abstract: CYP105AS1 is a cytochrome P450 from Amycolatopsis orientalis that catalyzes monooxygenation of compactin to 6-epi-pravastatin. For fermentative production of the cholesterol-lowering drug pravastatin, the stereoselectivity of the enzyme needs to be inverted, which has been… read more here.
Keywords: production; cytochrome p450; pravastatin; stereoselectivity ... See more keywords
Influence of the Trans/Cis Conformer Ratio on the Stereoselectivity of Peptidic Catalysts.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.7b06194
Abstract: Trans/cis isomerization of Xaa-Pro bonds is key for the structure and function of several enzymes. In recent years, numerous versatile peptidic catalysts have been developed that bear Xaa-Pro amide bonds. Due to the many degrees… read more here.
Keywords: bond; peptidic catalysts; ratio; trans cis ... See more keywords
Evolutionary insights into the stereoselectivity of imine reductases based on ancestral sequence reconstruction
Sign Up to like & getrecommendations! Published in 2024 at "Nature Communications"
DOI: 10.1038/s41467-024-54613-3
Abstract: The stereoselectivity of enzymes plays a central role in asymmetric biocatalytic reactions, but there remains a dearth of evolution-driven biochemistry studies investigating the evolutionary trajectory of this vital property. Imine reductases (IREDs) are one such… read more here.
Keywords: evolution; ancestral sequence; imine reductases; biochemistry ... See more keywords