Two‐Tier Screening Platform for Directed Evolution of Aminoacyl–tRNA Synthetases with Enhanced Stop Codon Suppression Efficiency
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DOI: 10.1002/cbic.201700039
Abstract: Genetic code expansion through amber stop codon suppression provides a powerful tool for introducing non‐proteinogenic functionalities into proteins for a broad range of applications. However, ribosomal incorporation of noncanonical amino acids (ncAAs) by means of… read more here.
Keywords: stop codon; platform; codon; efficiency ... See more keywords
A homozygous stop codon in HORMAD2 in a patient with recurrent digynic triploid miscarriage
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DOI: 10.1002/mgg3.2402
Abstract: Recurrent miscarriage (RM) affects 1% to 5% of couples trying to conceive. Despite extensive clinical and laboratory testing, half of the RM cases remain unexplained. We report the genetic analysis of a couple with eight… read more here.
Keywords: stop codon; codon hormad2; hormad2 patient; homozygous stop ... See more keywords
Premature Stop Codon at Residue 101 within HIV-1 Rev Does Not Influence Viral Replication of Clade BC but Severely Reduces Viral Fitness of Clade B
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DOI: 10.1007/s12250-019-00179-0
Abstract: HIV-1 Rev is an accessory protein that plays a key role in nuclear exportation, stabilization, and translation of the viral mRNAs. Rev of HIV-1 clade BC often shows a truncation of 16 AAs due to… read more here.
Keywords: premature stop; rev; clade; stop codon ... See more keywords
Conformational Control of Translation Termination on the 70S Ribosome.
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DOI: 10.1016/j.str.2018.04.001
Abstract: Translation termination ensures proper lengths of cellular proteins. During termination, release factor (RF) recognizes a stop codon and catalyzes peptide release. Conformational changes in RF are thought to underlie accurate translation termination. However, structural studies… read more here.
Keywords: translation termination; stop codon; termination; codon recognition ... See more keywords
Enhanced Codon-Anticodon Interaction at In-Frame UAG Stop Codon Improves the Efficiency of Non-Natural Amino Acid Mutagenesis.
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DOI: 10.1021/acschembio.1c00782
Abstract: The introduction of non-natural amino acids into proteins through the stop codon readthrough methodology has been used to design proteins for diverse applications. However, this method suffers from low yields of the modified protein, as… read more here.
Keywords: natural amino; interaction; codon; stop codon ... See more keywords
A Cell-Free Gene Expression Platform for Discovering and Characterizing Stop Codon Suppressing tRNAs.
Sign Up to like & getrecommendations! Published in 2023 at "ACS chemical biology"
DOI: 10.1021/acschembio.3c00051
Abstract: Non-canonical amino acids (ncAAs) can be incorporated into peptides and proteins to create new properties and functions. Site-specific ncAA incorporation is typically enabled by orthogonal translation systems comprising a stop codon suppressing tRNA (typically UAG),… read more here.
Keywords: stop codon; cell free; codon suppressing; free gene ... See more keywords
VIRMA mediates preferential m6A mRNA methylation in 3′UTR and near stop codon and associates with alternative polyadenylation
Sign Up to like & getrecommendations! Published in 2018 at "Cell Discovery"
DOI: 10.1038/s41421-018-0019-0
Abstract: N6-methyladenosine (m6A) is enriched in 3′untranslated region (3′UTR) and near stop codon of mature polyadenylated mRNAs in mammalian systems and has regulatory roles in eukaryotic mRNA transcriptome switch. Significantly, the mechanism for this modification preference… read more here.
Keywords: stop codon; near stop; utr near; m6a ... See more keywords
Translational error in mice increases with ageing in an organ-dependent manner
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DOI: 10.1038/s41467-025-57203-z
Abstract: The accuracy of protein synthesis and its relation to ageing has been of long-standing interest. To study whether spontaneous changes in the rate of ribosomal error occur as a function of age, we first determined… read more here.
Keywords: age; codon readthrough; stop codon; organ dependent ... See more keywords
Engineering a genomically recoded organism with one stop codon
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DOI: 10.1038/s41586-024-08501-x
Abstract: The genetic code is conserved across all domains of life, yet exceptions have revealed variations in codon assignments and associated translation factors1, 2–3. Inspired by this natural malleability, synthetic approaches have demonstrated whole-genome replacement of… read more here.
Keywords: stop codon; codon; single codon; genomically recoded ... See more keywords
Evolution of mitochondrial RNA editing sites and stop codon-lacking transcripts in angiosperms
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DOI: 10.1038/s42003-025-08418-9
Abstract: While the evolution of mitochondrial protein-coding genes in angiosperms has been extensively studied, the post-transcriptional RNA processing remains under-explored. Leveraging newly generated and public RNA-seq data from twenty representative angiosperms, we systematically inferred the gain-and-loss… read more here.
Keywords: stop codon; rna editing; rna; lacking transcripts ... See more keywords
Transcript-specific induction of stop codon readthrough using a CRISPR-dCas13 system
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DOI: 10.1038/s44319-024-00115-8
Abstract: Stop codon readthrough (SCR) is the process where translation continues beyond a stop codon on an mRNA. Here, we describe a strategy to enhance or induce SCR in a transcript-selective manner using a CRISPR-dCas13 system.… read more here.
Keywords: dcas13 system; crispr dcas13; stop codon; dcas13 ... See more keywords