Two‐Tier Screening Platform for Directed Evolution of Aminoacyl–tRNA Synthetases with Enhanced Stop Codon Suppression Efficiency
Sign Up to like & getrecommendations! Published in 2017 at "ChemBioChem"
DOI: 10.1002/cbic.201700039
Abstract: Genetic code expansion through amber stop codon suppression provides a powerful tool for introducing non‐proteinogenic functionalities into proteins for a broad range of applications. However, ribosomal incorporation of noncanonical amino acids (ncAAs) by means of… read more here.
Keywords: stop codon; platform; codon; efficiency ... See more keywords
Premature Stop Codon at Residue 101 within HIV-1 Rev Does Not Influence Viral Replication of Clade BC but Severely Reduces Viral Fitness of Clade B
Sign Up to like & getrecommendations! Published in 2019 at "Virologica Sinica"
DOI: 10.1007/s12250-019-00179-0
Abstract: HIV-1 Rev is an accessory protein that plays a key role in nuclear exportation, stabilization, and translation of the viral mRNAs. Rev of HIV-1 clade BC often shows a truncation of 16 AAs due to… read more here.
Keywords: premature stop; rev; clade; stop codon ... See more keywords
Conformational Control of Translation Termination on the 70S Ribosome.
Sign Up to like & getrecommendations! Published in 2018 at "Structure"
DOI: 10.1016/j.str.2018.04.001
Abstract: Translation termination ensures proper lengths of cellular proteins. During termination, release factor (RF) recognizes a stop codon and catalyzes peptide release. Conformational changes in RF are thought to underlie accurate translation termination. However, structural studies… read more here.
Keywords: translation termination; stop codon; termination; codon recognition ... See more keywords
Enhanced Codon-Anticodon Interaction at In-Frame UAG Stop Codon Improves the Efficiency of Non-Natural Amino Acid Mutagenesis.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.1c00782
Abstract: The introduction of non-natural amino acids into proteins through the stop codon readthrough methodology has been used to design proteins for diverse applications. However, this method suffers from low yields of the modified protein, as… read more here.
Keywords: natural amino; interaction; codon; stop codon ... See more keywords
A Cell-Free Gene Expression Platform for Discovering and Characterizing Stop Codon Suppressing tRNAs.
Sign Up to like & getrecommendations! Published in 2023 at "ACS chemical biology"
DOI: 10.1021/acschembio.3c00051
Abstract: Non-canonical amino acids (ncAAs) can be incorporated into peptides and proteins to create new properties and functions. Site-specific ncAA incorporation is typically enabled by orthogonal translation systems comprising a stop codon suppressing tRNA (typically UAG),… read more here.
Keywords: stop codon; cell free; codon suppressing; free gene ... See more keywords
VIRMA mediates preferential m6A mRNA methylation in 3′UTR and near stop codon and associates with alternative polyadenylation
Sign Up to like & getrecommendations! Published in 2018 at "Cell Discovery"
DOI: 10.1038/s41421-018-0019-0
Abstract: N6-methyladenosine (m6A) is enriched in 3′untranslated region (3′UTR) and near stop codon of mature polyadenylated mRNAs in mammalian systems and has regulatory roles in eukaryotic mRNA transcriptome switch. Significantly, the mechanism for this modification preference… read more here.
Keywords: stop codon; near stop; utr near; m6a ... See more keywords
'Stop' in protein synthesis is modulated with exquisite subtlety by an extended RNA translation signal.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemical Society transactions"
DOI: 10.1042/bst20180190
Abstract: Translational stop codons, UAA, UAG, and UGA, form an integral part of the universal genetic code. They are of significant interest today for their underlying fundamental role in terminating protein synthesis, but also for their… read more here.
Keywords: stop codon; translation; protein synthesis; stop ... See more keywords
Split aminoacyl-tRNA synthetases for proximity-induced stop codon suppression.
Sign Up to like & getrecommendations! Published in 2023 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2219758120
Abstract: Synthetic biology tools for regulating gene expression have many useful biotechnology and therapeutic applications. Most tools developed for this purpose control gene expression at the level of transcription, and relatively few methods are available for… read more here.
Keywords: stop codon; codon suppression; gene expression;
Developing DMD therapeutics: a review of the effectiveness of small molecules, stop-codon readthrough, dystrophin gene replacement, and exon-skipping therapies
Sign Up to like & getrecommendations! Published in 2021 at "Expert Opinion on Investigational Drugs"
DOI: 10.1080/13543784.2021.1868434
Abstract: ABSTRACT Introduction Duchenne muscular dystrophy (DMD) is an X-linked recessive disorder caused by mutations in the dystrophin (DMD) gene. Most patients die from respiratory failure or cardiomyopathy. There are significant unmet needs for treatments for… read more here.
Keywords: exon skipping; codon readthrough; gene replacement; stop codon ... See more keywords
Delayed Seroconversion to HTLV-II Is Associated with a Stop-Codon Mutation in the pol Gene.
Sign Up to like & getrecommendations! Published in 2017 at "AIDS research and human retroviruses"
DOI: 10.1089/aid.2016.0286
Abstract: A known HIV-1-positive intravenous drug user was found to be human T cell lymphoma/leukemia virus-II (HTLV-II) DNA positive by polymerase chain reaction but seronegative in a screening ELISA. He was consistently DNA positive but took… read more here.
Keywords: htlv; mutation; stop codon; mutation pol ... See more keywords
HLA‐DQA1*05:01:11, a novel HLA‐DQA1 allele with an ochre stop codon
Sign Up to like & getrecommendations! Published in 2022 at "HLA"
DOI: 10.1111/tan.14941
Abstract: The DQA1*05:01:11 allele shows the TAA (UAA) stop codon instead of the common DQA1 TGA (UGA) stop codon. read more here.
Keywords: dqa1; hla dqa1; hla; stop codon ... See more keywords