Transport Assays for Sterol-Binding Proteins: Stopped-Flow Fluorescence Methods for Investigating Intracellular Cholesterol Transport Mechanisms of NPC2 Protein.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6875-6_9
Abstract: In this chapter we describe the use of stopped flow fluorescence spectroscopy to analyze the kinetic mechanisms of protein mediated cholesterol transfer to, from, and between model membranes. These assays allow for the detection of… read more here.
Keywords: stopped flow; flow fluorescence; transport; cholesterol ... See more keywords
Stopped-Flow NMR and Quantitative GPC Reveal Unexpected Complexities for the Mechanism of NHC-Catalyzed Lactide Polymerization
Sign Up to like & getrecommendations! Published in 2017 at "Macromolecules"
DOI: 10.1021/acs.macromol.6b02139
Abstract: Stopped-flow NMR spectroscopy provides the first direct, in situ observation of lactide epimerization during polymerization with the N-heterocyclic carbene organocatalyst 1,3-dimesitylimidazol-2-ylidene (IMes). Hexad analysis of the polymer microstructure using 13C NMR spectroscopy supports a chain-end-controlled… read more here.
Keywords: lactide polymerization; polymerization; stopped flow; mechanism ... See more keywords
3D Capillary-Driven Paper-Based Sequential Microfluidic Device for Electrochemical Sensing Applications.
Sign Up to like & getrecommendations! Published in 2019 at "ACS sensors"
DOI: 10.1021/acssensors.8b01574
Abstract: This article describes the device design and fabrication of two different configurations (flow-through and stopped-flow) of a sequential fluid delivery platform on a microfluidic paper-based device. The developed device is capable of storing and transporting… read more here.
Keywords: complex assay; paper based; capillary driven; stopped flow ... See more keywords
Kinetics of complexation of V(v), U(vi), and Fe(iii) with glutaroimide-dioxime: studies by stopped-flow and conventional absorption spectroscopy.
Sign Up to like & getrecommendations! Published in 2017 at "Dalton transactions"
DOI: 10.1039/c7dt01597f
Abstract: Uranium extracted from seawater is a promising source of uranium for nuclear energy, and the extraction technology using polymer sorbents has been shown to be feasible. However, improving selectivity for uranium over other metals, notably… read more here.
Keywords: stopped flow; glutaroimide dioxime; flow conventional; spectroscopy ... See more keywords
Automated stopped-flow library synthesis for rapid optimisation and machine learning directed experimentation
Sign Up to like & getrecommendations! Published in 2022 at "Chemical Science"
DOI: 10.1039/d2sc03016k
Abstract: For the discovery of new candidate molecules in the pharmaceutical industry, library synthesis is a critical step, in which library size, diversity, and time to synthesise are fundamental. In this work we propose stopped-flow synthesis… read more here.
Keywords: reaction; machine learning; synthesis; stopped flow ... See more keywords
Multi-Step Unfolding and Rearrangement of α-Lactalbumin by SDS Revealed by Stopped-Flow SAXS
Sign Up to like & getrecommendations! Published in 2020 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2020.00125
Abstract: Interactions between proteins and surfactants are both of fundamental interest and relevant for applications in food, cosmetics and detergency. The anionic surfactant sodium dodecyl sulfate (SDS) denatures essentially all proteins. Denaturation typically involves a number… read more here.
Keywords: protein; ala; stopped flow; protein surfactant ... See more keywords
Stopped Flow of Glycerol Induces the Enhancement of Adsorption and Aggregation of HRP on Mica
Sign Up to like & getrecommendations! Published in 2023 at "Micromachines"
DOI: 10.3390/mi14051024
Abstract: Glycerol is a usable component of heat-transfer fluids, and is thus suitable for the use in microchannel-based heat exchangers in biosensors and microelectronic devices. The flow of a fluid can lead to the generation of… read more here.
Keywords: aggregation; hrp; glycerol; stopped flow ... See more keywords